Purified anti-Synaptotagmin-6 Antibody

Pricing & Availability
Clone
N270/47 (See other available formats)
Other Names
Synaptotagmin VI, Synaptotagmin 6, SYT6
Isotype
Mouse IgG1, κ
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Product Citations
publications
N270slash47_PURE_Synaptotagmin-6_WB_082718
Western blot of purified anti-Synaptotagmin-6 antibody (clone N270/47). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N270slash47_PURE_Synaptotagmin-6_WB_082718
    Western blot of purified anti-Synaptotagmin-6 antibody (clone N270/47). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
853901 25 µg $70
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853902 100 µg $140
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Description

Synaptotagmin-6 (SYT6) is a member of the synaptotagmin family, which consists of 13 membrane trafficking proteins that contain a N-terminal transmembrane region, and two cytoplasmic C2 domains. It has been reported that SYT6 plays an important role in Ca2+-dependent exocytosis of secretory vesicles, thus being an essential part of the neurotransmitter release machinery. SYT6 also mediates exocytosis in sperm acrosomal reaction via similar Ca2+-dependent mechanism.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein corresponding to amino acids 246-333 (cytoplasmic C2A domain) of mouse Synaptotagmin-6.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The double bands observed in WB may correspond to the phosphorylation status of SYT61.

In-house evaluation shows that this antibody does not work in IHC.

Application References

(PubMed link indicates BioLegend citation)
  1. Roggero CM, et al. 2005. Dev. Biol. 285:422-35.
RRID
AB_2750179 (BioLegend Cat. No. 853901)
AB_2750180 (BioLegend Cat. No. 853902)

Antigen Details

Structure
Synaptotagmine-6 is a 511 amino acid protein with a molecular mass of ~57 kD.
Distribution

Tissue Distribution: Mainly expressed in the brain (olfactory bulb)

Cellular Distribution: Cytosol and plasma membrane

Function
SYT6 is involved in Ca2+-dependent exocytosis of secretory vesicles.
Interaction
SYT6 forms homodimer and may also form heterodimer with STY3, SYT7, SYT9, and SYT10. SYT6 also interacts with STX1A, STX1B, and STX2.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References
  1. Mikoshiba K et al. 1999. Chem. Phys. Lipids. 98:59.
  2. Wong YH, et al. 2015. Proc. Natl. Acad. Sci. USA. 112:E4475.
Gene ID
148281 View all products for this Gene ID 60565 View all products for this Gene ID
UniProt
View information about Synaptotagmin-6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/27/2018

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