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- Clone
- M029F6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Erythropoietin
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot analysis of 50 ng of recombinant human EPO (lane 1) and 50 ng of recombinant mouse EPO (lane 2) using anti-mouse EPO antibody (clone M029F6).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 660302 | 100 µg | $288 | ||||
EPO is a glycoprotein that is glycosylated up to 40-60%, which causes its molecular weight to vary from 30-34 kD depending on the carbohydrate percentage. Hypoxia induces erythropoiesis, and hypoxia inducible factor (HIF) is directly involved in EPO expression. HIF-Prolyl hydroxylase (HIF-PH) is a sensor of levels of iron, oxygen, and metabolic activity. High levels of HIF protein induces EPO production in the kidneys and liver, and mobilizes iron to support erythropoiesis. EPO has been used in the treatment of anemia associated with chronic kidney disease, treatment of cancer patients on chemotherapy, and antiviral HIV therapy.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse EPO
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product does not cross react with human EPO.
- RRID
-
AB_2563470 (BioLegend Cat. No. 660302)
Antigen Details
- Structure
- 166 amino acid, predicted molecular weight of approximately 18.6 kD, migrates at approximately 30-34 kD as a result of its glycosylation.
- Distribution
-
EPO is primarily expressed in the kidneys (70-90% of the total EPO) and liver. EPO is expressed by hepatocytes, cortical interstitial fibroblasts in ischemic kidneys, astrocytes, and neurons under hypoxia.
- Function
- EPO is the major regulator of differentiation, proliferation, and survival of erythroid progenitors. EPO expression is induced by hypoxia, HIF, and other transcription factors. EPO has a possible role in neuroprotection.
- Interaction
- Erythroid progenitor cells.
- Ligand/Receptor
- EpoR (homodimer).
- Cell Type
- Fibroblasts, Neurons
- Biology Area
- Cell Biology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Shoemaker CB, et al. 1986. Mol. Cell. Biol. 6:849.
2. McDonald JD, et al. 1986. Mol. Cell. Biol. 6:842.
3. Chin K, et al. 2000. Brain Res. Mol. Brain Res. 81:29.
4. Snow JW, et al. 2009. J. Biol. Chem. 284:29310.
5. Kassouf MT, et al. 2010. Genome Res. 2010:20:1064.
6. Paliege A, et al. 2010. Kidney Int. 77:312.
7. Sinclair AM, et al. 2010. Blood 115:4264.
8. Alnaeeli M, et al. 2012. Anat. Res. Int. 2012:953264. - Gene ID
- 2056 View all products for this Gene ID
- UniProt
- View information about EPO on UniProt.org
Related FAQs
Other Formats
View All EPO Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-EPO | M029F6 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-EPO
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