LEGENDplex™ Human Growth Factor Panel (13-plex) w/ FP

Pricing & Availability
Regulatory Status
RUO
Other Names
Angiopoietin-2, EGF, EPO, FGF-basic, G-CSF, GM-CSF, HGF, M-CSF, PDGF-AA, PDGF-BB, SCF, TGF-α, VEGF
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Product Citations
publications
Cat # Size Price Quantity Save
740180 100 tests $1761
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Description

Growth factors activate cell proliferation and differentiation, and regulate many cellular processes including angiogenesis, hematopoiesis, tumorigenesis, metabolism, inflammation, and tissue repair. They could also be potential therapeutic targets and disease biomarkers. The accurate measurement of growth factors is critical for a better understanding of disease progression and related cellular processes.

The LEGENDplex™ Human Growth Factor Panel is a bead-based multiplex assay, using fluorescence-encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 human growth factors: Angiopoietin-2 (Ang-2), EGF, EPO, FGF-basic, G-CSF, GM-CSF, HGF, M-CSF, PDGF-AA, PDGF-BB, SCF, TGF-α, and VEGF. This panel provides higher sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on serum, plasma and cell culture supernatant samples.

A LEGENDplex™ Mix and Match system is available to customize assays for specificities within this growth factor panel.

Product Details
View Current Manual (pdf)

Kit Contents

Kit Contents
  • Setup Beads: PE Beads (1 mL)
  • Setup Beads: Raw Beads (2 mL)
  • Capture Beads
  • Detection Antibody (3.5 mL)
  • Standard Cocktail, Lyophilized (1 vial)
  • SA-PE (3.5 mL)
  • Matrix B5, Lyophilized (1 vial)
  • Assay Buffer (25 mL)
  • Wash Buffer (25 mL)
  • Filter Plate (1 Plate)
  • Plate Sealers (4 sheets)

Please note: Lots manufactured before September 2021 may include a different version of the “Matrix B.” There is no change in the protocol. Simply use the Matrix reagent you received as suggested in the current manual.

Product Details

Verified Reactivity
Human
Application

Multiplex


Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.
Product Citations
  1. Bhandi S, et al. 2021. Saudi J Biol Sci. 28:3559. PubMed
  2. Bhandi S, et al. 2021. J Pers Med. 11:. PubMed
  3. Yusuf AM, et al. 2021. J Cell Physiol. Online ahead of print. PubMed
  4. Gonçalves MF, et al. 2020. Tissue Eng Part A. 26:769. PubMed
  5. Shekatkar MR, et al. 2022. J Clin Transl Res. 8:323. PubMed
  6. Gupta A, et al. 2022. Life Sci. 304:120703. PubMed
  7. Raj AT, et al. 2021. Molecules. 26:. PubMed
  8. Raj AT, et al. 2021. Saudi J Biol Sci. 28:6556. PubMed
  9. Dregalla RC, et al. 2022. Stem Cells Int. 2022:8259888. PubMed
  10. Kulshrestha A, et al. 2022. Sci Rep. 12:7325. PubMed
  11. Cloer C, et al. 2021. PLoS One. 16:e0259732. PubMed
Materials Not Included
  • For a full list, please read the manual
  • Flow Cytometer
  • Pipettes and Tips
  • Reagent Reservoirs for Multichannel Pipettes
  • Polypropylene Microfuge Tubes
  • Vortex Mixer
  • Sonicator
  • Plate Shaker
  • A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
  • Manual

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 5    Revision Date: 03/28/2023

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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