- Other Names
- Lactadherin Pre-coated ELISA Kit, Milk fat globule-EGF factor 8 (MFG-E8) Pre-coated ELISA Kit
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- Product Citations
|438107||1 Pre-coated Plate||$350|
Lactadherin, a novel adhesion molecule, also known as MFG-E8, is a widely distributed glycoprotein (~50 kD) that has been found in macrophages, dendritic cells, vascular smooth muscle cells, astrocytes, microglia, breast carcinioma cells, and also in breast milk and in and around blood vessels. Lactadherin contains an N-terminal EGF-like motif with an inserted RGD cell adhesion sequence; it also contains a C-terminal sequence sharing homology with the discoidin family of lectin domains, including the phospholipid-binding domains of blood clotting factors V and VIII. Lactadherin promotes RGD-dependent cell attachment of a variety of cell types, but most notably of cells that express the integrin heterodimer, alpha v β 3. The C1/C2 domain binds specifically to phosphotidylserine. Mounting evidence has shown that Lactadherin plays a far more important role than just protecting the newborn, a function originally identified. Lactadherin functions as an anticoagulant by blocking phosphatidylserine-containing membrane sites for blood coagulation proteins. It is also involved in cell death by recognizing phosphatidylserine that is exposed on apoptotic cells. Lactadherin is also crucial in VEGF-dependent neovascularization and has been identified as an important target for the modulation of neovascularization. In addition, Lactadherin was identified as a novel target for radioimmunotherapy in breast cancer and is also important in the initiation and/or progression of Alzheimer’s disease.
BioLegend’s LEGEND MAX™ Mouse Lactadherin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a mouse Lactadherin specific polyclonal antibody is pre-coated on a 96-well strip-well plate.
This Kit is specifically designed for the quantitation of Mouse Lactadherin from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and cost-effective.
- Kit Contents
- Anti-Mouse Lactadherin Pre-coated 96-well Strip Microplate
- Mouse Lactadherin Dectection Antibody
- Mouse Lactadherin Standard
- Avidin-HRP D
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- 53.1 pg/mL
- Standard Range
- 93.8-6,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Shaker
- Polypropylene vials
- Cell Sources
- Macrophages, dendritic cells, vascular smooth muscle cells, astrocytes, microglia, breast carcinioma cells
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Gene ID
- 17304 View all products for this Gene ID
- View information about Lactadherin on UniProt.org
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?
We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.