LEGEND MAX™ Mouse Lactadherin ELISA Kit

Pricing & Availability
Other Names
Lactadherin Pre-coated ELISA Kit, Milk fat globule-EGF factor 8 (MFG-E8) Pre-coated ELISA Kit
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Lactadherin_Mouse_052511
  • Lactadherin_Mouse_052511
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438107 1 Pre-coated Plate $350
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Description

Lactadherin, a novel adhesion molecule, also known as MFG-E8, is a widely distributed glycoprotein (~50 kD) that has been found in macrophages, dendritic cells, vascular smooth muscle cells, astrocytes, microglia, breast carcinioma cells, and also in breast milk and in and around blood vessels. Lactadherin contains an N-terminal EGF-like motif with an inserted RGD cell adhesion sequence; it also contains a C-terminal sequence sharing homology with the discoidin family of lectin domains, including the phospholipid-binding domains of blood clotting factors V and VIII. Lactadherin promotes RGD-dependent cell attachment of a variety of cell types, but most notably of cells that express the integrin heterodimer, alpha v β 3. The C1/C2 domain binds specifically to phosphotidylserine. Mounting evidence has shown that Lactadherin plays a far more important role than just protecting the newborn, a function originally identified. Lactadherin functions as an anticoagulant by blocking phosphatidylserine-containing membrane sites for blood coagulation proteins. It is also involved in cell death by recognizing phosphatidylserine that is exposed on apoptotic cells. Lactadherin is also crucial in VEGF-dependent neovascularization and has been identified as an important target for the modulation of neovascularization. In addition, Lactadherin was identified as a novel target for radioimmunotherapy in breast cancer and is also important in the initiation and/or progression of Alzheimer’s disease.

BioLegend’s LEGEND MAX™ Mouse Lactadherin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a mouse Lactadherin specific polyclonal antibody is pre-coated on a 96-well strip-well plate.

This Kit is specifically designed for the quantitation of Mouse Lactadherin from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and cost-effective.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Mouse Lactadherin Pre-coated 96-well Strip Microplate
  • Mouse Lactadherin Dectection Antibody
  • Mouse Lactadherin Standard
  • Avidin-HRP D
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Mouse
Application

ELISA

Sensitivity
53.1 pg/mL
Standard Range
93.8-6,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Macrophages, dendritic cells, vascular smooth muscle cells, astrocytes, microglia, breast carcinioma cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
17304 View all products for this Gene ID
UniProt
View information about Lactadherin on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 2    Revision Date: 09/11/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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