Go-ChIP-Grade™ Purified Armenian Hamster IgG Isotype Ctrl Antibody

Pricing & Availability
Clone
HTK888 (See other available formats)
Isotype
Armenian Hamster IgG
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Product Citations
publications
HTK888_GoChIP_IgG_Antibody_1_082619
Chromatin Immunoprecipitations (ChIP) were done with fixed and sonicated chromatin samples from Jurkat cells. ChIP was performed with 3 µg of chromatin and either 1.2 µg of Go-ChIP-Grade™ Purified Armenian Hamster IgG Isotype Control Antibody (clone HTK888, in black) or no antibody (beads control, in gray). Protein G beads were used to isolate immune complexes. The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for active (human GAPDH promoter and human RPL30 exon 3) and inactive (human α-Satellite repeats) loci. The amount of immunoprecipitated DNA in each sample is represented as percentage to the total amount of input chromatin, which is equivalent to 100%.
  • HTK888_GoChIP_IgG_Antibody_1_082619
    Chromatin Immunoprecipitations (ChIP) were done with fixed and sonicated chromatin samples from Jurkat cells. ChIP was performed with 3 µg of chromatin and either 1.2 µg of Go-ChIP-Grade™ Purified Armenian Hamster IgG Isotype Control Antibody (clone HTK888, in black) or no antibody (beads control, in gray). Protein G beads were used to isolate immune complexes. The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for active (human GAPDH promoter and human RPL30 exon 3) and inactive (human α-Satellite repeats) loci. The amount of immunoprecipitated DNA in each sample is represented as percentage to the total amount of input chromatin, which is equivalent to 100%.
Cat # Size Price Quantity Avail. Save
400971 25 µg $40
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400972 100 µg $85
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Description

This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
FC, ICFC - Validated
WB, IP, ICC, IF - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Application Notes

The HTK888 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 400916) as negative control. For in vivo studies or functional assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) as a negative control.

Application References

(PubMed link indicates BioLegend citation)
  1. Lesley R, et al. 2006. P. Natl. Acad. Sci. USA 103:10717.
  2. Yu R, et al. 2006. Obesity 14:1353.
  3. Yang JH, et al.2005. Rheumatology(Oxford). 44:1245. PubMed
  4. Mina-Osorio P, et al. 2008. J. Leukocyte Biol. 84:448. PubMed
  5. Shen H, et al. 2009. J. Am Soc Nephrol. 20:1032. PubMed

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Go To Top Version: 1    Revision Date: 05/07/2019

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