- Clone
- 13A3-1 (See other available formats)
- Other Names
- Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells starved overnight and then treated with IL-6 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT3 Phospho (Tyr705) (Clone 13A3-1), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (#400101), or C) competitor’s ChIP-grade Purified anti-STAT3 Phospho (Tyr705) Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IRF1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin. -
Total cell lysate from PC3 (lane 1, 15 µg), untreated HepG2 (lane 2, 15 µg), HepG2 treated with 10 ng/ml of IL-6 for 15 minutes (lane 3, 15 µg) and HeLa (lane 4, 15 µg) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with 1 µg/ml Purified anti-STAT3 Phospho (Tyr705) antibody (clone 13A3-1). Proteins were visualized using an HRP Goat anti-mouse IgG (clone Poly4053) antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control. -
15 µg of total cell lysate from untreated NIH3T3 (lane 1) and NIH3T3 treated with 100 ng/ml of IL-6 for 30 minutes (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-STAT3 Phospho (Tyr705) antibody (clone 13A3-1). Proteins were visualized using an HRP Goat anti-mouse IgG (clone Poly4053) antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 651015 | 25 µg | $75 | ||||
| 651016 | 100 µg | $235 | ||||
Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.
Product DetailsProduct Details
- Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH conjugated modified synthetic peptide
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ChIP - Quality tested
WB - Validated - Recommended Usage
-
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 by volume. For Western blotting the suggested working dilution is 0.5 µg per ml antibody dilution buffer for each mini-gel.
It is recommended that the reagent to be titrated for optimal performance before each experiment.
- Application Notes
-
The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting.
- RRID
-
AB_2715801 (BioLegend Cat. No. 651015)
AB_2715802 (BioLegend Cat. No. 651016)
Antigen Details
- Structure
- STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
- Distribution
-
Ubiquitous.
- Function
- STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases.
- Cell Sources
- Cytoplasm. Translocate to nucleus in response to tyrosine phosphorylation.
- Cell Type
- Embryonic Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors
- Molecular Family
- Phospho-Proteins
- Antigen References
-
1. Akira S, et al. 1994. Cell 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145. - Regulation
- The small GTPase Rac1 binds and regulates activity of STAT3.
- Gene ID
- 6774 View all products for this Gene ID
- UniProt
- View information about STAT3 Phospho on UniProt.org
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