- 12A4A35 (See other available formats)
- Other Names
- Interferon regulatory factor-3 (IRF-3)
- Mouse IgG2b, κ
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IRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB protein binding to dsRNA-activated factor 1, a complex which activates the transcription of genes under control of ISRE. The phosphorylated IRF-3 is under negative regulation by ubiquitin-mediated degradation. Research indicates that it plays a critical role in stimulated TLR-mediated IL-27 p28 gene expression.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Partial IRF-3 recombinant protein (200-427 a.a.)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ChIP - Quality tested
WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. It is recommended that the reagent to be titrated for optimal performance before each experiment.
- Application Notes
25 µg, 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution.
AB_2721589 (BioLegend Cat. No. 655703)
AB_2721590 (BioLegend Cat. No. 655704)
- 427 amino acids, predicted molecular weight of 47 kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding.
Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.
- IRF-3 is a constitutively expressed, latent transcription factor that plays a pivotal role in type I IFN response.
- CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Lin R, et al. 1999. Mol. Cell Biol. 19:959.
2. Hiscott J, et al. 2007. J. Biol. Chem. 282:15325.
3. Molle C, et al. 2010. J. Immunol. 184:1784.
- Gene ID
- 3661 View all products for this Gene ID
- View information about IRF3 on UniProt.org
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