Go-ChIP-Grade™ Purified anti-human Ikaros Antibody

Pricing & Availability
Clone
16B5C71 (See other available formats)
Other Names
IK1, IKZF1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
16B5C71_GoChIP_Ikaros_Antibody_050918
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-human Ikaros (Clone 16B5C71, Cat. No. 687103) or B) equal amount of Purified Mouse IgG2a, κ Isotype Control Antibody (Clone MG2a-53, Cat. No. 401501) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human Hes1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 16B5C71_GoChIP_Ikaros_Antibody_050918
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-human Ikaros (Clone 16B5C71, Cat. No. 687103) or B) equal amount of Purified Mouse IgG2a, κ Isotype Control Antibody (Clone MG2a-53, Cat. No. 401501) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human Hes1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 16B5C71_PURE_Ikaros_Antibody_WB_120717
    Total lysates (15 µg protein) from Jurkat and HeLa cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL Go-ChIP-Grade™ Purified anti-ikaros Antibody, clone 16B5C71(upper), or 1:2000 diluted anti-GAPDH Antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-ikaros Antibody, or 1:3000 diluted donkey anti-Rabbit-IgG secondary antibody conjugated to HRP for anti-GAPDH Antibody. Lane M: Molecular weight ladder; 1: Jurkat; 2: HeLa.
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687103 25 µg $75
 
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687104 100 µg $235
 
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Description

Ikaros, also known as IKZF1, belongs to a family of zinc-finger DNA-binding proteins that regulate lymphocyte differentiation. It has the ability to form homo- and heterodimers with other members of the Ikaros family, which include Aiolos and Helios. Ikaros can be found on a majority of fetal and adult hematopoietic cell types such as T- and B-cells, natural killer cells (NKs), monocytes/macrophages, and dendritic cells. Ikaros is a tumor suppressor and its absence or reduced expression has been reported in several leukemias.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB - Validated 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:200 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

There are eight splicing variants with predicted MW 58, 53, 48(2), 43, 41, 31, 24 KDa. This antibody might recognize some of the isoforms.

RRID
AB_2721587 (BioLegend Cat. No. 687103)
AB_2721588 (BioLegend Cat. No. 687104)

Antigen Details

Structure
Zinc-finger DNA binding protein, homo/heterodimer, 57.5 kD
Distribution

Fetal and adult hematopoietic cells.

Function
Regulates lymphocyte differentiation.
Interaction
DNA.
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Wargnier A, et al. 1995. Proc. Natl. Acad. Sci. USA 92:6930.
2. Tonnelle C, et al. 2009. Mol. Immunol. 46:1736.
3. Wang H, et al. 2014. Pediatr. Blood Cancer 61:2230.

Gene ID
10320 View all products for this Gene ID
UniProt
View information about Ikaros on UniProt.org

Related FAQs

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