Alexa Fluor® 647 anti-human/mouse MRP1 (ABCC1) Antibody

Pricing & Availability
Clone
QCRL-2 (See other available formats)
Other Names
ABC29, ABCC, GS-X, MRP, Multidrug resistance-associated protein 1, LTC4 transporter, leukotriene C(4) transporter
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
QCRL2_AF647_MRP1_ABCC1_Antibody
H69AR cells were treated with the True-Nuclear™ Transcription Factor Buffer Set and then intracellularly stained with MRP1 (clone QCRL-2) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
  • QCRL2_AF647_MRP1_ABCC1_Antibody
    H69AR cells were treated with the True-Nuclear™ Transcription Factor Buffer Set and then intracellularly stained with MRP1 (clone QCRL-2) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
370203 25 tests $125
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370204 100 tests $295
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Description

MRP1 is a 190 kD integral membrane phosphoglycoprotein. It is a member of the human ATP-binding cassette transporter multidrug resistance protein1 (MRP1), encoded by ABCC1. MRP1 is expressed in most normal tissues, with higher levels in the testes, lung, skin, small intestine, colon, kidney, placenta, peripheral blood mononuclear cells, and skeletal muscle. ABCC1 encodes plasma membrane proteins that have important physiological transport functions and mediate a variety of xenobiotics against their concentration gradients. MRP1 plays an important role in the extrusion of drugs from the cell and their over expression can be a cause of failure of anticancer and antimicrobial chemotherapy.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
QCRL-2 was raised against membranes prepared from the human small cell lung cancer cell line, H69AR, which highly over expresses MRP1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

QCRL-2 detects a discontinuous conformation-dependent epitope wholly contained in NBD1 of MRP1. The epitope for QCRL-2 has been mapped to aa 617-858. QCRL-2 is reported to be an effective and specific inhibitor of MRP1 activity when used in transport assays with inside-out membrane vesicles. QCRL-1, QCRL-2, QCRL-3, and QCRL-4 each bind distinct non-overlapping epitopes.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Product Citations
  1. Lin JR 2018. eLife. 7: e31657. PubMed
Publication Library
RRID
AB_2566664 (BioLegend Cat. No. 370203)
AB_2566665 (BioLegend Cat. No. 370204)

Antigen Details

Structure
190 kD integral membrane phosphoglycoprotein.
Distribution
Testes, lung, skin, small intestine, colon, kidney, placenta, peripheral blood mononuclear cells, and skeletal muscle. Cytoplasm / plasma membrane.
Function
Extrusion of drugs from the cell.
Biology Area
Immunology
Antigen References

1. Cole SP, et al. 2014. Annu. Rev. Pharmacol. Toxicol. 54:95.
2. Hipfner DR, et al. 1999. J. Biol. Chem. 274(22):15420.
3. Filipits M, et al. 1999. Anticancer Res. 6:5043.
4. Wright SR, et al. 1998. Clin. Cancer Res. 4:2279.
5. Hipfner DR, et al. 1998. Br. J. Cancer 78(9):1134.
6. Filipits M, et al. 1997. Leukemia 11(7):1073.
7. Loe DW, et al. 1997. Mol. Pharmacol. 6:1034-41.
8. Hipfner DR, et al. 1996. Cancer Res. 56:3307.
9. Filipits M, et al. 1996. Clin. Cancer Res. 2(7):1231.
10. Almquist KC, et al. 1995. Cancer Res. 55(1):102.

Gene ID
4363 View all products for this Gene ID
UniProt
View information about MRP1 (ABCC1) on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12/13/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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