Alexa Fluor® 594 anti-TARP γ2 (Stargazin) Antibody

Pricing & Availability
Clone
N245/1 (See other available formats)
Other Names
MRD10, TARP gamma-2, Voltage-dependent calcium channel gamma-2 subunit, TARP gamma-2, transmembrane AMPAR regulatory protein gamma-2, neuronal voltage-gated calcium channel gamma-2 subunit
Isotype
Mouse IgG2a
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Product Citations
publications
N245-1_AF594_TARPg2_Stargazin_ICC_041719
SH-SY5Y cells were fixed with 4% paraformaldehyde (PFA) and blocked with 5% FBS. Then the cells were stained with 5 µg/ml Alexa Fluor® 594 anti-TARP γ2 (Stargazin) Antibody (red). Nuclei were counterstained with DAPI and are shown in blue. The image was captured with a 60X objective.
  • N245-1_AF594_TARPg2_Stargazin_ICC_041719
    SH-SY5Y cells were fixed with 4% paraformaldehyde (PFA) and blocked with 5% FBS. Then the cells were stained with 5 µg/ml Alexa Fluor® 594 anti-TARP γ2 (Stargazin) Antibody (red). Nuclei were counterstained with DAPI and are shown in blue. The image was captured with a 60X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
681604 100 µg $325
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Description

Transmembrane AMPAR regulatory protein (TARP) γ2, also known as voltage-dependent calcium channel γ2 (CACNG2), MRD10, and stargazin, is a subunit of L-type calcium channels that stabilizes inactive (closed state) calcium channels. Stargazin has an essential role in learning due its role in AMPAR transport.

Encoded by CACNG2 gene, TARP γ2 (Stargazin), a 36-kD protein, is a brain-specific transmembrane AMPA receptor regulatory protein that modulates the trafficking and ion channel kinetics of glutamate AMPA receptors. It is also a putative subunit of neuronal voltage-gated calcium channels. Six TARP isoforms, γ-2 (stargazin), γ-3, γ-4, γ-5, γ-7, and γ-8, have distinct though partially overlapping patterns of expression in the central nervous system (CNS). Stargazin not only mediates AMPA receptor trafficking, but also shapes synaptic responses by slowing channeling deactivation and desensitization. It alters AMPA receptor kinetics by increasing the rate of channel opening. Disrupting the interaction of stargazin ectodomain with hippocampal AMPA receptors alters the shape of synaptic responses, which establishes a crucial function for stargazin in controlling the efficacy of synaptic transmission in the brain.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 203-323 (cytoplasmic C-terminus) of rat TARPγ2.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in Immunocytochemistry (ICC).

ICC Positive Control: Human SH-SY5Y cell line  

RRID
AB_2566481 (BioLegend Cat. No. 681604)

Antigen Details

Structure
323 amino acids with a predicted molecular weight of 36 kD.
Distribution

Plasma membrane.

Function
Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties.
Interaction
DLG4/PSD-95 and DLG1/SAP97, GOPC (by similarity), GRIA1, found in a complex with GRIA1, GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG3, CACNG4, CACNG5, CACNG7, and CACNG8 (by similarity).
Ligand/Receptor
Acts as an auxiliary subunit for AMPA-selective glutamate receptors.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Letts VA, et al. 1998. Nat. Genet. 4:340.
2. Chen L, et al. 2000. Nature 408:936.
3. Fukaya M, et al. 2005. Neurosci. Res. 53:376.
4. Nissenbaum J, et al. 2010. Genome Res. 9:1180.
5. Rigby M, et al. 2015. J. Neurosci. 35:4203.
6. Hamdan FF, et al. 2011. Am. J. Hum. Genet. 88:306.

Gene ID
84347 View all products for this Gene ID
UniProt
View information about TARP Gamma2/Stargazin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/30/2015

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