Application: |
Flow cytometry |
Cells used: |
Murine lung or lymph node |
Brief Protocol: |
Collect cells, wash and stain in FACS buffer. Block with FcBlock. Stain at 1:200 4oC for 20 minutes. Detect by flow cytometry. Fixable in PFA if desired. |
Results Summary: |
Consistent staining, populations are more of a smear that a distinct population but that is likely due to the antigen not the antibody or product formulation and makes sense based on what I would predict for CD44 expression. |
Additional Notes: |
Will use again. No issues noted. |
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