Product: Brilliant Violet 605™ anti-human CD196 (CCR6)

Catalog No.: 353419

Magalie Collet, lab Manager, RA III the Jackson Lab

Brilliant Violet 605™ anti-human CCR6
Overall:	Product Quality:	Ease of Use:
Bright antibody for nice TH or TfH separation.

Experimental Design

Application:	Flow Cytometry
Cells used:	Human PBMC
Brief Protocol:	500K PBMC freshly isolated were stained with an antibody cocktail containing CCR6 Brilliant Violet 605™(clone G034E3) for 15min at RT at dark in a 96V plate. Dilution used 3/100 from working antibody concentration of 20ug/ml. Cells were then washed twice and span at 2000 rpm for 4 min and acquired on LSRII.
Results Summary:	Bright antibody for nice TH or TfH separation.
Additional Notes:	Stock concentration of 100ug/ml was diluted to a working concentration of 20ug/ml for a final concentration of 1ug/ml during staining.

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