Product: Recombinant Human IFN-γ (carrier-free) |
Catalog No.: 570204 |
Jeremy Bechelli, Mr. University of Texas Medical Branch |
Great for Positive Controls! (Recombinant Human IFN-γ) | |||
Overall: | Product Quality: | Ease of Use: | |
We use this cytokine as a positive control for autophagy induction and inflammasome activation in macrophages. |
Experimental Design |
Application: | Positive control for autophagy induction. |
Cells used: | Human macrophages and endothelial cells |
Brief Protocol: | Treat cells with 100 ng of IFNg for 24 hours for the induction of autophagy. |
Results Summary: | We see increased LC3-II by western blot and decreased p62 levels. |
Additional Notes: |
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