Product: Brilliant Violet 421™ anti-human CD56 (NCAM)

Catalog No.: 318328

Anne Rissiek, Dr. Universitätsklinikum Hamburg-Eppendorf (UKE)

Anti-human CD56 Brilliant Violet 421™
Overall:	Product Quality:	Ease of Use:
Phenotyping NK cells.

Experimental Design

Application:	FACS
Cells used:	Human PBMCs
Brief Protocol:	1. 1 mio fresh isolated human PBMCs in 100 µl FACS buffer plus 50 µl antibody cocktail (in FACS Buffer), incubate for 30 min at RT. 2. Lyse red good cells by adding 1 ml Lysis Buffer, incubate for 10 min at RT, wash 2x with PBS, 400 g, 5 min, trash supernatant. 3. Resuspend cells in 200-300 µl FACS Buffer, FACS analysis. The CD56 antibody was titrated and applied at a final dilution of 1:300.
Results Summary:	Very good discrimination of NK cells.
Additional Notes:	Gating strategy: Lymphocytes --> Single cells --> not T (CD3+) an not B (CD19+) --> CD56/CD16.

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Publication Status This data has not been published.

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