Product: Pacific Blue™ anti-mouse CD103

Catalog No.: 121418

Robert Yuan, Graduate Student Stanford

CD103+ Cells in the Small Intestine are Stained Well with the Antibody
Overall:	Product Quality:	Ease of Use:
The antibody distinguishes small intestinal CD103+ cells well.

Experimental Design

Application:	Flow Cytometry
Cells used:	Small intestinal cells
Brief Protocol:	Small intestine was harvested from a mouse and digested using a collagenase-based digestion protocol to obtain a single-cell suspension. The cells were then put through a 40%/75% Percoll gradient, with the cells at the interface taken for further staining. Cells were then blocked with Fc Block, stained with antibodies, and read on a flow cytometer.
Results Summary:	CD103+ cells were stained clearly with the antibody.
Additional Notes:	Cells were pre-gated on DAPI- cells.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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