Product: APC/Cyanine7 anti-human CD45 |
Catalog No.: 368516 |
Ryan Sowell, PhD Student Rush University Medical Center |
CD45 Staining for Flow Cytometry | |||
Overall: | Product Quality: | Ease of Use: | |
Works well for staining of previously frozen human PBMCs. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Primary CD8 T cells |
Brief Protocol: | Thawed and stained previously frozen primary human peripheral blood mononuclear cells. CD45 APC/Cyanine7 stained at 1:200 dilution for 30 minutes at 4oC. Washed and fixed in BioLegend's Fixation buffer. |
Results Summary: | CD45 separation is clear and background is minimal for freshly thawed cells. |
Additional Notes: |
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