Application: |
Flow cytometry |
Cells used: |
Thymocytes and splenocytes |
Brief Protocol: |
Half of the cells were incubated at 56ËšC for 10 min and the other half were kept on ice
- Extracellular staining - Wash in PBS 1X - Wash in binding buffer - Add Annexing-V FITC alone or in combination with 7-AAD in 100 ul of binding buffer per million of cells - Wait 15 min at RT and Analyze by FACS |
Results Summary: |
The best stain index is obtained with 0.3125 ul of Annexin V FITC in 100 ul per million of cells |
Additional Notes: |
Very efficient Annexin product at low price. |
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