Product: Purified anti-Neurofilament H (NF-H), Phosphorylated |
Catalog No.: 801601 |
SK Ha, Postdoc fellow NIH/NINDS |
Review of anti-Neurofilament H (SMI-31) | |||
Overall: | Product Quality: | Ease of Use: | |
This is an excellent antibody. |
Experimental Design |
Application: | Formalin-fixed paraffin embedded tissue |
Cells used: | common marmoset and human tissue |
Brief Protocol: | 1. deparaffin and rehydrate the tissue 2. antigen retrieval by citrate buffer pH 6.0 3. blocking 4. 1st Ab,1:100, 2 hour RT 5. washing 6. 2nd Ab by Leica Power Vision 7. washing 8. development by DAB and hematoxylin counterstaining |
Results Summary: | Stained well at thick and thin axons. |
Additional Notes: |
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