Product: FITC anti-mouse/human CD44

Catalog No.: 103006

Joseph Lownik, MD/PhD Student VCU SOM

FITC anti-mouse/human CD44 for the Identification of Activated T Cells
Overall:	Product Quality:	Ease of Use:
FITC anti-CD44 was used to identify activated T cells in a mouse house dust mite model of airway hyperresponsiveness.

Experimental Design

Application:	Flow Cytometry
Cells used:	Mouse lung single cell suspensions
Brief Protocol:	Mice were challenged with house dust mite for two weeks. Lungs were isolated and digested to a single cell suspension. T cells were restimulated using anti-CD3 coated plates for 4 hours in the presence of Monensin. Cells were fixed and permeabilized using Biolegend's Intracellular Cytokine Staining Buffer. Cells were stained for 60 minutes at room temperature with PE anti-mouse IL4 1:200 dilution of FITC anti-mouse CD44. Data was analyzed using an LSR Fortessa.
Results Summary:	FITC anti-CD44 was successfully used for the identification of activated T cells.
Additional Notes:	Gated on CD4+ T cells.

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Publication Status This data has not been published.

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