Product: PE anti-human CD274 (B7-H1, PD-L1) |
Catalog No.: 329705 |
Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital |
Staining PBMC with anti-human B7-H1 PE | |||
Overall: | Product Quality: | Ease of Use: | |
Anti-B7-H1 PE works well on PBMC. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Human PBMC |
Brief Protocol: | PBMC were isolated from blood by centrifugation over Ficoll. PBMC were washed twice in PBS and stained for 15 minutes at room temperature with the recommended dilution of anti-B7-H1 PE in 100uL FACS buffer (PBS w/2% FBS and 0.09% sodium azide). Cells were washed twice with 2mL FACS buffer and analyzed on a BD LSR II. |
Results Summary: | B7-H1 staining was as expected (filled histogram), as compared to unstained cells (dotted line). |
Additional Notes: |
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