Product: Biotin anti-mouse CD31

Catalog No.: 102504

Robert Yuan, Graduate Student Stanford

Immunofluorescence Using Anti-Mouse CD31
Overall:	Product Quality:	Ease of Use:
Immunofluorescence staining using the product as a primary antibody to visualize blood vessels in mouse intestine.

Experimental Design

Application:	Immunofluorescence
Cells used:	Pre-fixed, frozen mouse small intestine
Brief Protocol:	A 7 micron section of a frozen, PFA pre-fixed mouse small intestine was washed in 0.1% tritonX-100 and blocked in 5% goat serum in 1% BSA in PBS. Section was then stained with the product overnight at 1:100 at 4 degrees C. Secondary antibody used was goat anti-rat Alexa Fluor 594 at 1:200. Section was then DAPI stained, mounted, and imaged with a Zeiss confocal microscope.
Results Summary:	The product stained blood vessel-like structures in the mouse intestine cleanly.
Additional Notes:	Some staining of rounder myeloid-like cells was also evident. DAPI is shown in blue. CD31 staining is shown in red.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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