Product: Pacific Blue™ anti-human CD19

Catalog No.: 302223

Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital

Staining PBMC with anti-human CD19 Pacific Blue™
Overall:	Product Quality:	Ease of Use:
Anti-CD19 Pacific Blue™ is not the best choice for staining PBMC.

Experimental Design

Application:	Flow cytometry
Cells used:	Human PBMC
Brief Protocol:	PBMC were isolated from blood by centrifugation over Ficoll. PBMC were washed twice in PBS and stained for 15 minutes at room temperature with the recommended dilution of anti-CD19 Pacific Blue™ in 100uL FACS buffer (PBS w/2% FBS and 0.09% sodium azide). Cells were washed twice with 2mL FACS buffer and analyzed on a BD LSR II.
Results Summary:	Pacific Blue™ CD19 staining was dim, with positive cells barely resolvable from negative cells.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

Technical Service Notes

Pacific Blue™ is a relatively dim fluor (a 2 on our scale of 1-5). In certain situations, they enhanced brightness of Brilliant Violet™ 421 (5 on a scale of 1-5) may be more suitable for certain samples. For troubleshooting help, please don't hesitate to contact tech@biolegend.com.

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