Extensive Assessment of Cytokine Production Following Immune Cell Stimulation on the NovoCyte Penteon using the LEGENDplex™ Bead-based Multiplex Assay

In this application note, learn how you can use bead-based, multiplex flow cytometry assays to simultaneously measure an array of cytokines in samples from infected patients or patients undergoing therapeutics.

 

BioLegend LEGENDplex™️ panels follow similar principles as an ELISA, providing accurate quantification of concentrations with high detection sensitivity, and a broader dynamic range than traditional methods. In combination with intracellular cytokine staining, one can detect specific cytokines produced in different cell types with the use of cell surface markers. Determining the concentration of a secreted cytokine, as well as the cell type producing said cytokine, provides more comprehensive information on the immune cell response to stimuli.

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