The study of antigen-specific T cells is crucial to a number of different fields, including vaccine development, cancer research, and antigen analysis. To aid in the identification of these T cells, BioLegend is proud to launch Flex-T™ MHC Tetramers, a novel technology licensed from Sanquin Blood Supply Foundation. Flex-T™ provides flexibility by allowing you to load your peptide of interest into the MHC binding site.
Flex-T™ features:
  • Flexible peptide loading
  • Two color combination capability
  • High throughput screening capable
  • High specificity
  • Ease of use
  • Affordability

Flex-T™ Procedure

Flex-T™ Data

A) CD8+ T Cells previously gated on lymphocytes (FSC vs SSC) and 7-AAD negative events, the exclusion cocktail contains Alexa Fluor® 700 anti- CD4, CD19, CD14, and CD16. B) Antigen-specific CD8+ T Cells, gated as described, detected with Flex-T™ tagged with PE and APC. HLA-A*11:01 Flex-T™ is loaded with EBV peptide (IVTDFSVIK).

Flex-T™ Products

Class I Allele Biotinylated Monomer

Flex-T™ HLA-A*01:01 Monomer UVX
Flex-T™ HLA-A*02:01 Monomer UVX
Flex-T™ HLA-A*03:01 Monomer UVX
Flex-T™ HLA-A*11:01 Monomer UVX
Flex-T™ HLA-B*07:02 Monomer UVX
Flex-T™ HLA-B*08:01 Monomer UVX

Accessories

Flex-T™ HLA Class I ELISA Control
Purified Streptavidin
HRP anti-human Β2-microglobulin
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