- 17D9/CLEC-2 (See other available formats)
- Other Names
- CLEC1B, CLEC1B1
- Rat IgG2b, κ
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- Product Citations
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CLEC-2, also known as CLEC1B and CLEC1B1, is C-type lectin-like receptor with a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM). CLEC-2 is highly expressed on resting platelets and megakaryocytes and at a lower levels on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. The first ligand that was identified for CLEC-2 was the snake venom toxin rhodocytin; podoplanin has also been identified as an endogenous ligand for CLEC-2. Exposure of platelets to rhodocytin leads tyrosine phosphorylation of the ITAM-like motif and Syk-dependent platelet activation. CLEC-2 also functions as an activation receptor on monoyctes and neutrophils to induce phagocytosis and proinflammatory cytokine production.Product Details
- Antibody Type
- Host Species
- RBL-2H3 cells stably expressing HA-tagged mouse CLEC2.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for relevant formats) include: Western blotting1.
- Application References
- Mourpo-Sß D, et al. 2011. Eur. J. Immunol. 41:3040. (WB)
- Product Citations
AB_2562240 (BioLegend Cat. No. 146102)
- Type II transmembrane protein with a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM)
Platelets, megakaryocytes, granulocytes, monocytes, macrophages, and dendritic cells
- Involved in platelet aggregation and secretion as well as neutrophil phagocytosis
- Venom toxin, rhodocytin, and podoplanin
- Cell Type
- Dendritic cells, Granulocytes, Macrophages, Megakaryocytes, Monocytes, Platelets, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Antigen References
1. Boulaftali Y, et al. 2013. J. Clin. Invest. 123:908.
2. Manne BK, et al. 2013. J. Biol. Chem. 288:7717.
3. Acton SE, et al. 2012. Immunity. 37:276.
4. Finney BA, et al. 2012. Blood 119:1747.
5. Kerrigan AM, et al. 2009. J. Immunol. 182:4150.
- Gene ID
- 56760 View all products for this Gene ID
- View information about CLEC-2 on UniProt.org
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