Purified anti-Histone H3 Nonmethyl (Lys9) Antibody (Previously Covance catalog# MMS-5083)

Pricing & Availability
Clone
9B1-2G6 (See other available formats)
Other Names
Histone H3.1, histone H3/a, histone 1, H3/a, H3 histone family, member A
Previously
Covance Catalog# MMS-5083
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
9B1-2G6_purified_Histone-H3-Nonmethyl_Antibody_1_010219
Western blot of purified anti-Histone H3 Nonmethyl (Lys9) antibody (clone 9B1-2G6). Lane 1: Molecular weight marker; Lane 2: 30 µg of HeLa nuclear cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 9B1-2G6_purified_Histone-H3-Nonmethyl_Antibody_1_010219
    Western blot of purified anti-Histone H3 Nonmethyl (Lys9) antibody (clone 9B1-2G6). Lane 1: Molecular weight marker; Lane 2: 30 µg of HeLa nuclear cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 9B1-2G6_purified_Histone-H3-Nonmethyl_Antibody_2_010219
    IHC staining of purified anti-Histone H3 Nonmethyl (Lys9) antibody (clone 9B1-2G6) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
815701 100 µL 187€
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Description

Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, non-methylated at Lysine-9.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissues, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564795 (BioLegend Cat. No. 815701)

Antigen Details

Structure
Expected MW: 17 kD
Biology Area
Cell Biology, Neuroscience, Signal Transduction
Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone H3 (Lys9) on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 01/02/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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