- B34 (See other available formats)
- Other Names
- Green Fluorescent Protein
Covance Catalog# MMS-118P
- Mouse IgG1
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- Product Citations
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Green Fluorescent Protein (GFP) is a single chain 238 amino acid protein derived from the jellyfish Aequoria victoria. GFP emits a bright green fluorescence when illuminated with UV or blue light and is a useful tool for cellular and developmental biologists. Unlike other bioluminescent molecules, GFP does not require additional proteins, substrates or co-factors for its activity.Product Details
- Green Fluorescent Protein Tag
- Antibody Type
- Host Species
- Antibody B34, generated against purified wild type GFP, offers an alternitive for detecting GFP and GFP variants including enhanced/mutant GFP.
- Phosphate-buffered solution + 0.03% Thimerosal.
- The antibody was purified by affinity chromatography.
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:5,000*
- Application Notes
This antibody is effective in immunoblotting (WB) of GFP labeled proteins.
*Predicted MW = 27 kD
- Application References
- Pecot MY, Malhotra V. 2004. Cell. 116:99.
- Kessels MM, et al. 2001. J Cell Biol. 153:351.
- Gerdes HH, Kaether C. 1996. FEBS Lett. 389:44.
- Davis I, et al. 1995. Dev Biol. 170:726.
- Chalfie M. 1995. Photochem Photobio. 62:651.
- Stearns T. 1995. Curr Biol. 5:262.
- Chalfie M, et al. 1994. Science. 263:802.
- Lavy M, et al. 2007. Curr. Biol. 17:947. (WB)
- Hobl B, et al. 2013. Protein Expr. Purif. 10:32. (WB)
- Zhang Y, et al. 2007. J. Biol. Chem. 282:13022. (WB) PubMed
- Asante D, et al. 2013. J. Cell. Sci. 126:5189. (WB) PubMed
- Oh Y, et al. 2013. Mol Biol Cell. 24:1305. (WB) PubMed
- Wloka C, et al. 2013. J. Cell. Biol. 200:271. (WB)
- Redfern RE, et al. 2010. Protein Sci. 19:1948. (WB) PubMed
- Product Citations
AB_2734671 (BioLegend Cat. No. 902605)
AB_2565021 (BioLegend Cat. No. 902601)
AB_2565022 (BioLegend Cat. No. 902602)
- Biology Area
- Cell Biology
- Gene ID
- View information about GFP on UniProt.org
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