- 1B7 (See other available formats)
- Regulatory Status
- Other Names
- RNA binding protein fox-1 homolog 3, fox-1 homolog C, neuronal nuclei, hexaribonucleotide binding protein 3
Covance Catalog# SIG-39860
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
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Fox3 is one of a family of 3 mammalian Fox homologues. Fox was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X". The Fox protein and its 3 mammalian homologues are all about 46 kD proteins, each of which includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. Fox3 is a protein which has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei. The 1B7 antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of astrocytes and other kinds of non-neuronal cells. This Fox3/NeuN antibody is therefore an excellent marker of neuronal cells.Product Details
- Human, Mouse, Rat
- Antibody Type
- Host Species
- Antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli.
- Phosphate-buffered solution + 10mM sodium azide.
- The antibody was purified by affinity chromatography.
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
IHC-P - Quality tested
WB - Verified
ICC, IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 1.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.01 - 0.05 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The double band observed in the immunoblot corresponds to the two NeuN isoforms produced by alternative splicing with expected molecular weights of 46 kD and 48 kD.
- Application References
- Herculano-Houzel S and Lent R. 2005. J Neurosci. 25:2518-21. (ICC)
- Kim KK, et al. 2009. J Biol Chem. 284:31052-31061. (WB, ICC, IHC-P)
- Underwood JG, et al. 2005. Mol Cell Biol. 25:10005-10016. (IHC-F, WB)
- Product Citations
AB_2734601 (BioLegend Cat. No. 834502)
AB_2564991 (BioLegend Cat. No. 834501)
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