Purified anti-α-1-Acid Glycoprotein Antibody

Pricing & Availability
Clone
BL28596 (See other available formats)
Regulatory Status
RUO
Other Names
a1AGP, ORM1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_1_052119
Western blot of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596). Lane 1: Molecular weight marker; Lane 2: 5 µg of human liver lysate; Lane 3: 5 µg of mouse liver lysate; Lane 4: 5 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_1_052119
    Western blot of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596). Lane 1: Molecular weight marker; Lane 2: 5 µg of human liver lysate; Lane 3: 5 µg of mouse liver lysate; Lane 4: 5 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_2_052119
    IHC staining of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_3_052119
    IHC staining of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596) on formalin-fixed paraffin-embedded mouse liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_4_052119
    IHC staining of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596) on formalin-fixed paraffin-embedded rat liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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859601 25 µg 85€
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Description

α-1-Acid Glycoprotein (α1AGP) is a key acute phase plasma protein synthesized by hepatocytes and functions as a transport protein in the blood stream. The levels of α1AGP become elevated in acute inflammation and therefore it is classified as an acute-phase reactant.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant α-1-Acid Glycoprotein (a1AGP) expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810733 (BioLegend Cat. No. 859601)

Antigen Details

Structure
α-1-Acid Glycoprotein is a 201 amino acid protein with a predicted molecular mass of 24 kD and observed molecular mass of 40 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol and nucleus

Function
α-1-Acid Glycoprotein is an acute phase plasma protein which functions as transport protein in the blood stream.
Interaction
Synthetic drugs, cytosolic Ca2+, immune response
Cell Type
Mesenchymal cells, Neurons
Biology Area
Immuno-Oncology, Immunology
Molecular Family
Adaptor Proteins, Innate Immune Signaling
Antigen References
  1. Luo Z, et al. 2015. J Physiol Biochem.  71(2):329.
  2. Xu YF, et al. 2018. Comb Chem High Throughput Screen. 21(10):755.
Gene ID
5004 View all products for this Gene ID 18405 View all products for this Gene ID 24614 View all products for this Gene ID
UniProt
View information about alpha-1-Acid Glycoprotein on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/17/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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