PE anti-human LGR5 (GPR49) Antibody

Pricing & Availability
Clone
SA222C5 (See other available formats)
Regulatory Status
RUO
Other Names
G-Protein coupled receptor 49 (GPR49), G-Protein coupled receptor 67 (GPR67), FEX, HG38
Isotype
Mouse IgG2a, κ
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Product Citations
publications
SA222C5_PE_LGR5_Antibody_041917
BaF3 cells transfected with human LGR5 were stained with anti-human LGR5 PE (clone SA222C5, closed histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • SA222C5_PE_LGR5_Antibody_041917
    BaF3 cells transfected with human LGR5 were stained with anti-human LGR5 PE (clone SA222C5, closed histogram) or mouse IgG2a, κ PE isotype control (open histogram).
See PE spectral data
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373803 25 tests 100.00€
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373804 100 tests 220.00€
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Description

LGR5, also known as GPR49, is a 100 KD protein. It is a member of GPCR class A orphan receptor proteins which binds R-spondin and does not activate through heterotrimeric G protein like other GPCR. It triggers the Wnt signaling pathway downstream. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human LGR5 transfectant cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
RRID
AB_2686987 (BioLegend Cat. No. 373803)
AB_2686988 (BioLegend Cat. No. 373804)

Antigen Details

Structure
G-protein coupled receptor (GPCR) superfamily
Distribution

Muscle, placenta, spinal cord, brain and particularly as a biomarker of adult stem cells in certain tissues.

Function
Triggers Wnt signaling pathway.
Interaction
Wnt pathway
Ligand/Receptor
R-spondins (RSpo 1,2,3, and 4)
Biology Area
Immunology
Antigen References

1. Hsu Sy, et al. 1998. Mol. Endocrinol. 12:1830.
2. Barker N, et al. 2007. Nature. 449:1003.
3. Morita H, et al. 2004. Mol. Cell. Biol. 24(22):9736.
4. Arioka Y, et al. 2017. Stem Cell Res. 20:1.
5. Wang B, et al. 2016. Plos. One. 11:e0168904.
6. Garcia MI, et al. 2009. Dev. Biol. 331:58.

Gene ID
8549 View all products for this Gene ID
UniProt
View information about LGR5 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 04/19/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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