- Other Names
- Multiplex assay, Flow cytometry, Inflammation, Flex set, Cytokines
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- Product Citations
Cytokines are small soluble glycoproteins that act as extracellular signaling molecuels and meidate communications between cells. They regulate immunity, inflammation and hematopoiesis as well as cell growth, migration, development and differentiation. Cytokines act in large tightly regulated networks where both the production and action of one cytokine is affected by the behavior of other cytokines. The accurate measurement of the expression profile of cytokines in a sample is critical to complete and in-depth understanding of disease progression and immune responses.
The LEGENDplex™ Mouse Inflammation Panel is a multiplex assay by using fluorescence–encoded beads suitable for the analysis on various flow cytometers. This panel allows simultaneous quantification of 13 mouse cytokines, including IL-1α, IL-1β, IL-6, IL-10, IL-12p70, IL-17A, IL-23, IL-27, MCP-1, IFN-β, IFN-γ, TNF-α, and GM-CSF. Most of cytokines in this panel are produced by innate immune cells, linking the innate and adaptive immunity and/or bystander cells. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated by detecting expected changes in biological samples.
A LEGENDplex™ Mix and Match system is available to customize assays for specificities within this panel.
- Kit Contents
- Setup Beads 1: FITC Beads (1 ml)
- Setup Beads 2: PE Beads (1 ml)
- Setup Beads 3: Raw Beads (2 ml)
- Capture Beads
- Detection Antibody (3.5 ml)
- Standard Cocktail, Lyophilized (1 vial)
- SA-PE (3.5 ml)
- Matrix C, Lyophilized (1 vial)
- Assay Buffer (25 ml)
- Wash Buffer (25 ml)
- V-bottom Plate (1 plate)
- Plate Sealers (4 sheets)
- Data Analysis Software Dongle
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here.
- Product Citations
- Materials Not Included
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Plate Shaker
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Innate Immunity
- Molecular Family
- Gene ID
- 3553 View all products for this Gene ID 3439 View all products for this Gene ID 3440 View all products for this Gene ID 3458 View all products for this Gene ID 7124 View all products for this Gene ID 3569 View all products for this Gene ID 3576 View all products for this Gene ID 3586 View all products for this Gene ID 3592 View all products for this Gene ID 3593 View all products for this Gene ID 3605 View all products for this Gene ID 3606 View all products for this Gene ID 3593 View all products for this Gene ID 51561 View all products for this Gene ID 90865 View all products for this Gene ID
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.