GMP PE anti-human CD103 (Integrin αE) Antibody, CD103, Ber-ACT8

Pricing & Availability

Clone: Ber-ACT8 (See other available formats)
Workshop: V A067
Other Names: Integrin alpha E (ITGAE)
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

Ber-ACT8_GMP_PE_CD103_111323 — Typical result of PHA-stimulated (3 days) human peripheral blood lymphocytes stained either with Ber-ACT8 PE used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

Compare all formats See GMP PE spectral data

Cat #

Size

Price

Quantity Check Availability

Save

260330

100 tests

244€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

CD103 is a type I transmembrane glycoprotein also known as αE integrin, integrin αIEL chain, and human mucosal lymphocyte antigen 1. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. Treg cells express high level of CD103. Hairy cell leukemia has also been shown to express CD103. The expression of CD103 on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as an αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Product Details

Quality Statement This BioLegend’s GMP-RUO reagent is a fluorochrome-labeled monoclonal antibody single reagent for Research Use Only. Suitable for use in laboratory-developed multicolor flow cytometry tests. Single GMP-RUO products can be used in various multicolor flow cytometry combinations. Laboratory need to establish the GMP-RUO performance characteristics in combination with other reagents in normal and pathological samples.

Technical Data Sheet (pdf)

Product Details

Reactivity

Human

Reported Reactivity

Cynomolgus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

HTLV-1 induced human T cell line MAPS16

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

12 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: Western Blotting¹, immunoprecipitation¹, and immunohistochemical staining of frozen tissue sections¹.

Application References

Kruschwitz M, et al. 1991. J. Clin. Pathol. 44:636. (WB, IP, IHC-F)
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

RRID

AB_3083148 (BioLegend Cat. No. 260330)

Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

Batch-to-batch consistency
Material traceability
Documented procedures
Documented employee training
Equipment maintenance and monitoring records
Lot-specific certificates of analysis
Quality audits per ISO 13485:2016
QA review of released products

Antigen Details

Structure: Type I transmembrane glycoprotein, integrin family; can be cleaved into 150 kD and 25 kD chains; associated with β7 integrin
Distribution: Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells; expressed on hairy cell leukemia
Function: Retention and activation of CD103⁺ lymphocytes in the intestinal epithelium, regulation of tissue-specific T cell homing
Ligand/Receptor: E-Cadherin
Cell Targets: Integrin β7
Cell Type: Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Parker CM, et al. 1992. P. Natl. Acad. Sci. USA 89:1924.
2. Kruschwitz M, et al. 1991. J. Clin. Pathol. 44:636.
3. Schon MP, et al. 1999. J. Immunol. 162:6641.
4. Shaw SK, et al. 1994. J. Biol. Chem. 269:6016.
Gene ID: 3682 View all products for this Gene ID
UniProt: View information about CD103 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD103 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD103 (Integrin αE)	Ber-ACT8	FC,WB,IP,IHC-F
FITC anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Alexa Fluor® 488 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Alexa Fluor® 647 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE/Cyanine7 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Brilliant Violet 421™ anti-human CD103 (Integrin αE)	Ber-ACT8	FC
APC anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Brilliant Violet 605™ anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Biotin anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Brilliant Violet 711™ anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE/Dazzle™ 594 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PerCP/Cyanine5.5 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Brilliant Violet 785™ anti-human CD103 (Integrin αE)	Ber-ACT8	FC
APC/Cyanine7 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
TotalSeq™-A0145 anti-human CD103 (Integrin αE)	Ber-ACT8	PG
TotalSeq™-C0145 anti-human CD103 (Integrin αE)	Ber-ACT8	PG
TotalSeq™-B0145 anti-human CD103 (Integrin αE)	Ber-ACT8	PG
APC/Fire™ 750 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE/Fire™ 700 anti-human CD103 (Integrin αE) Antibody	Ber-ACT8	FC
TotalSeq™-D0145 anti-human CD103 (Integrin αE)	Ber-ACT8	PG
PE/Fire™ 640 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE anti-human CD103	Ber-ACT8	FC
FITC anti-human CD103	Ber-ACT8	FC
APC anti-human CD103	Ber-ACT8	FC
Spark YG™ 581 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Spark NIR™ 685 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Spark Blue™ 550 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE/Fire™ 810 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
GMP FITC anti-human CD103 (Integrin αE)	Ber-ACT8	FC
GMP PE anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Spark Red™ 718 anti-human CD103 (Integrin αE) (Flexi-Fluor™)	Ber-ACT8	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12/13/2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.