Loading...

FITC anti-human CD3 Antibody

Pricing & Availability
Clone
OKT3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
OKT3_FITC_053107
Human peripheral blood lymphocytes stained with OKT3 FITC
  • OKT3_FITC_053107
    Human peripheral blood lymphocytes stained with OKT3 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
317305 25 tests 23€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
317306 100 tests 44€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Li B, et al. 2005. Immunology 116:487.
  5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
  6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
  7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
  8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
  9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
Product Citations
  1. Guo X, et al. 2016. Mol Ther Methods Clin Dev. 3:15054. PubMed
  2. Zitti B, et al. 2017. Sci Rep. 10.1038/s41598-017-10403-0. PubMed
  3. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  4. Want MY, et al. 2019. Oncoimmunology. 8:e1586042. PubMed
  5. Lugo-Reyes SO, et al. 2021. J Clin Immunol. 41:1291. PubMed
  6. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  7. Clayton K, et al. 2014. J Immunol. 192:782. PubMed
  8. Shi Z, et al. 2021. Ann Clin Transl Neurol. 8:43. PubMed
  9. Catapano M, et al. 2020. J Invest Dermatol. 140:816. PubMed
  10. Wang Y, et al. 2019. J Transl Med. 17:93. PubMed
  11. Wu X, et al. 2020. Cancers (Basel). 12:. PubMed
  12. Moen IN, et al. 2021. Cell Death Discov. 7:36. PubMed
  13. Dai Q, et al. 2020. Front Immunol. 11:539654. PubMed
  14. Yamada E, et al. 2021. Gut Microbes. 13:1997292. PubMed
  15. Zhang B, et al. 2022. J Exp Med. 219:. PubMed
  16. Lin L, et al. 2018. Oncol Lett. 16:1259. PubMed
  17. Sun Y, et al. 2022. Nat Commun. 13:3916. PubMed
  18. Zhang Z, et al. 2021. Acta Pharm Sin B. 11:1965. PubMed
  19. Celik H, et al. 2021. Cancer Discov. 11:3126. PubMed
  20. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  21. Giesecke C, et al. 2017. Cytometry A. 10.1002/cyto.a.23250. PubMed
  22. Gillissen MA, et al. 2018. Blood. 131:131. PubMed
  23. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  24. Kim K, et al. 2019. Nat Microbiol. 1.586111111. PubMed
  25. Sperk M, et al. 2021. iScience. 24:102111. PubMed
  26. Nakasone H, et al. 2021. Commun Biol. 4:1177. PubMed
  27. Wu X, et al. 2021. Front Immunol. 12:731767. PubMed
  28. Jeyanathan M, et al. 2022. JCI Insight. 7:. PubMed
  29. Omer OS, et al. 2020. Methods Mol Biol. 2121:199. PubMed
  30. Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
  31. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  32. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  33. Agashe VV, et al. 2018. J Immunol. 201:772. PubMed
  34. Oyer JL, et al. 2022. Front Immunol. 13:861681. PubMed
  35. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  36. Aagaard K, et al. 2017. Sci Rep. 7:41389. PubMed
  37. Miyamae Y, et al. 2020. Cell Chemical Biology. 27(12):1573-1581.e3. PubMed
  38. Cheng J, et al. 2022. iScience. 25:103588. PubMed
  39. Tsai HI, et al. 2021. EMBO Mol Med. 13:e12834. PubMed
  40. Zhang W, et al. 2022. J Transl Med. 20:558. PubMed
  41. Zhao B, et al. 2013. PLoS One. 8:77708. PubMed
  42. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  43. Winkler C, et al. 2021. JCI Insight. 6:. PubMed
  44. Akusjärvi SS, et al. 2022. iScience. 25:103607. PubMed
  45. Zhou Z, et al. 2022. J Gastrointest Oncol. 13:732. PubMed
  46. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
  47. Shikhagaie M, et al. 2017. Cell Rep. 18(7):1761-1773. PubMed
  48. Gopalakrishnapillai A, et al. 2021. Cancers (Basel). 13:. PubMed
  49. Fierle JK, et al. 2021. Cell Reports Medicine. 2(8):100362. PubMed
  50. Sullivan KD et al. 2016. eLife. 5 pii: e16220. PubMed
  51. Ochi T, et al. 2011. Blood. 118:1495. PubMed
  52. Svensson Akusjärvi S, et al. 2022. Commun Biol. 5:357. PubMed
RRID
AB_571906 (BioLegend Cat. No. 317305)
AB_571907 (BioLegend Cat. No. 317306)

Antigen Details

Structure
Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
  2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
  3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Customers Also Purchased

FITC anti-human CD19
Clone: HIB19

PE anti-human CD45
Clone: HI30

PE anti-human CD3
Clone: OKT3

APC anti-human CD4
Clone: OKT4

Go To Top Version: 2    Revision Date: 06/12/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD3

  • FITC anti-human CD3

  • PE anti-human CD3

  • Alexa Fluor® 488 anti-human CD3

  • Alexa Fluor® 647 anti-human CD3

  • Pacific Blue™ anti-human CD3

  • APC anti-human CD3

  • Biotin anti-human CD3

  • Brilliant Violet 605™ anti-human CD3

  • Brilliant Violet 650™ anti-human CD3

  • Ultra-LEAF™ Purified anti-human CD3

  • Brilliant Violet 711™ anti-human CD3

  • Brilliant Violet 785™ anti-human CD3

  • Brilliant Violet 510™ anti-human CD3

  • PE/Cyanine7 anti-human CD3

  • PerCP/Cyanine5.5 anti-human CD3

  • PerCP anti-human CD3

  • Alexa Fluor® 700 anti-human CD3

  • APC/Cyanine7 anti-human CD3

  • Brilliant Violet 421™ anti-human CD3

  • PE/Dazzle™ 594 anti-human CD3

  • APC/Fire™ 750 anti-human CD3

  • GMP Ultra-LEAF™ Purified anti-human CD3 SF

  • PE/Cyanine5 anti-human CD3 Antibody
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account