Alexa Fluor® 660 anti-human CD3 Antibody, CD3, SK7

Pricing & Availability

Clone: SK7 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ
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Product Citations: publications

SK7_A660_CD3_Antibody_042920 — Human peripheral blood lymphocytes were stained with CD3 (clone SK7) Alexa Fluor® 660 (filled histogram). Open histogram represents unstained cells.

Compare all formats See Alexa Fluor® 660 spectral data

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Size

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344855

25 tests

100€

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344856

100 tests

212€

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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 660 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 660 has an excitation maximum of 663 nm, and a maximum emission of 690 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^4,5,8, immunofluorescent staining⁶, and Western blotting³.

Application References

Kan EA, et al. 1983. J. Immunol. 131:536.
Wood GS, et al. 1985. Am. J. Pathol. 120:371.
Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
Carbone A, et al. 1999. Blood 93:2319. (IHC)
Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
Rutjens E, et al. 2007. J. Immunol. 178:1702.
Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

RRID

AB_2860892 (BioLegend Cat. No. 344855)
AB_2860893 (BioLegend Cat. No. 344856)

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, during thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

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Certificate of Analysis

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Go To Top Version: 1 Revision Date: 04/29/2020

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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