Alexa Fluor® 647 anti-Syntaxin Antibody

Pricing & Availability
Clone
SP8 (See other available formats)
Regulatory Status
RUO
Other Names
Syntaxin-1A, neuron-specific antigen HPC-1
Isotype
Mouse IgG1
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Product Citations
publications
SP8_A647_Syntaxin_Antibody_1_121219
IHC staining of Alexa Fluor® 647 anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 200 µm
  • SP8_A647_Syntaxin_Antibody_1_121219
    IHC staining of Alexa Fluor® 647 anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 200 µm
  • SP8_A647_Syntaxin_Antibody_2_121219
    IHC staining of Alexa Fluor® 647 anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 200 µm
  • SP8_A647_Syntaxin_Antibody_3_121219
    IHC staining of Alexa Fluor® 647 anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 200 µm
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
827007 25 µg 108.00€
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827008 100 µg 220.00€
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Description

Syntaxin, also known as syntaxin-1A and neuron-specific antigen HPC-1, is part of the Q-SNARE protein family that participates in exocytosis. Syntaxins have a SNARE domain, an N-terminal regulatory domain, and a single C-terminal transmembrane domain. Syntaxin drives the spontaneous calcium-independent fusion of synaptic vesicles that contain v-SNARE proteins, and may form part of the fusion pore of exocytosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a crude synaptic preparation from a post-mortem human brain.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in immunohistochemistry (IHC-P) and western blotting (WB).

Application References
  1. Honer WG, et al. 1997. Neuroscience 78(1):99-110.
  2. Honer WG, et al. 1993. Brain Res. 609(1-2):9-20.
RRID
AB_2820129 (BioLegend Cat. No. 827007)
AB_2820130 (BioLegend Cat. No. 827008)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
6804 View all products for this Gene ID
UniProt
View information about Syntaxin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/12/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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