- Regulatory Status
- RUO
- Other Names
- Multiplex assay, flow cytometry, Bone Metabolism, Bone Metabolism Regulators, Formation, Ossification, Resorption, Osteoblast, Osteoclast, Osteoperosis, Rheumatoid Arthritis, Osteopetrosis, Paget’s disease, Rickets, Renal Osteodystrophy
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- Submit a Review
- Product Citations
- publications
Cat # | Size | Price |
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LEGENDplex™ Human Bone Metabolism Panel 1 Detection Antibodies will be used together with the following Mix and Match Capture Beads, Human Bone Metabolism Panel 1 Standard, and Buffer Set J to offer flexible customization within the panel (12-plex):
740660 LEGENDplex™ Human OPG Capture Bead A4, 13X
740661 LEGENDplex™ Human OPN Capture Bead A5, 13X
740663 LEGENDplex™ Human ALPL Capture Bead A7, 13X
740664 LEGENDplex™ Human ACP5 Capture Bead A8, 13X
740665 LEGENDplex™ Human Leptin Capture Bead A10, 13X
740666 LEGENDplex™ Human RANKL (TRANCE) Capture Bead B2, 13X
740053 LEGENDplex™ Human TNF-α Capture Bead B3, 13X
740667 LEGENDplex™ Human IL-6 Capture Bead B4, 13X
740668 LEGENDplex™ Human PTH Capture Bead B5, 13X
740669 LEGENDplex™ Human IL-1β Capture Bead B6, 13X
740670 LEGENDplex™ Human BMP-2 Capture Bead B7, 13X
740671 LEGENDplex™ Human DKK-1 Capture Bead B9, 13X
740658 LEGENDplex™ Human Bone Metabolism Panel 1 Standard
740623 LEGENDplex™ Buffer Set J (Set Up Beads, Buffers, Plate Sealers, SA-PE, Matrix, and Data Analysis Software Dongle)
740377 or 740379 Filter Plate or V-bottom Plate
The LEGENDplex™ Human Bone Metabolism Panel 1 is a multiplex bead-based assay panel, using fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 12 human proteins, including OPG, OPN, ALPL, ACP5, Leptin, RANKL, TNF-α, IL-6, PTH, IL-1β, BMP-2, and DKK-1. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated for use with serum, plasma, and tissue culture supernatants.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Application
-
Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here.
Antigen Details
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines, Enzymes and Regulators, Growth Factors
- Gene ID
- NA
- App Abbreviation (DOES NOT SHOW ON TDS):
- Multiplex - Component
Related Pages & Pathways
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Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
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If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
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Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
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The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
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LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
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Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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