BioLegend Direct-Blot
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The most common practice when performing a western blot is to use a secondary antibody to detect the proteins of interest. This is advantageous if there is a need to amplify the signal. However, if the target under study is abundant, and the quality of a directly conjugated primary antibody to Horse Radish Peroxidase (HRP) is high, there is really no advantage or need to use a secondary conjugated antibody. Thus, we are happy to introduce Direct-Blot™, primary antibodies directly conjugated to HRP, to help you speed up, simplify and reduce the cost of your experiment.
Summary of advantages:
  • Shorter protocol
  • Highly sensitive
  • Excellent stability
  • Easier to multiplex
  • Easier to combine with Immunoprecipitation
  • No potential background from HRP conjugated secondary antibodies
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