Ultra-LEAF™ Purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) Antibody

Pricing & Availability
Clone
N-GAD65 (See other available formats)
Regulatory Status
RUO
Other Names
Glutamate Decarboxylase 2, GAD65, Glutamate Decarboxylase 65 kD Isoform, GABA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N-GAD65_PURE_GAD65_Antibody_1_WB_052118
Western blot of purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) antibody (clone N-GAD65). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N-GAD65_PURE_GAD65_Antibody_1_WB_052118
    Western blot of purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) antibody (clone N-GAD65). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N-GAD65_PURE_GAD65_Antibody_2_052118
    IHC staining of purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) (clone N-GAD65) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N-GAD65_PURE_GAD65_Antibody_3_052118
    IHC staining of purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) (clone N-GAD65) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N-GAD65_PURE_GAD65_Antibody_4_052118
    IHC staining of purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) (clone N-GAD65) on formalin-fixed paraffin-embedded human pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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844503 100 µg 228,00€
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Description

GAD gene encodes one of the several forms of glutamic acid decarboxylase, which is identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid (GABA) from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. GAD65 targets presynaptic clusters in axons and exhibits relative dendritic exclusion. GAD65 synthesizes GABA for neurotransmission and is only necessary at nerve terminals and synapses. In order to aid in neurotransmission, GAD65 forms a complex with Heat Shock Cognate 70 (HSC70), cysteine string protein (CSP) and Vesicular GABA transporter (VGAT), which, as a complex, helps package GABA into vesicles for release during neurotransmission.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide immunogen corresponding to amino acids 4-22 of human GAD65.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry formalin-fixed paraffin-embedded tissues, a concentration range of 1.0 - 5.0 µg/ml is suggested.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation.1

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844503) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

This antibody demonstrates weaker reactivity to human compared to mouse GAD65/GAD2

Application References

(PubMed link indicates BioLegend citation)
  1. Hampe CS, et al. 2001. J. Neuroimmunol. 113:63. (IP, WB)
  2. Hampe CS, et al. 2007. Clin. Exp. Immunol. 148:72.
  3. Ziegler B, et al. 1996. Acta Diabetol. 33:225.
  4. Raju R, et al. 2005. J. Immunol. 175:7755.
Product Citations
RRID
AB_2565804 (BioLegend Cat. No. 844503)

Antigen Details

Structure
585 amino acids with a predicted molecular weight of 65 kD.
Distribution

Cytoplasm, cytoplasmic vesicle, golgi apparatus, plasma membrane, and marker of pre-synaptic terminals.

Function
Catalyzes production of GABA from L-glutamic acid.
Interaction
Taurine and hypotaurine metabolism, transmission across chemical synapses.
Cell Type
GABAergic Neurons
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Antigen References

1. Walls A, et al. 2011. J. of Cerebral Blood Flow & Metabolism 31:494.

Gene ID
2572 View all products for this Gene ID
UniProt
View information about Glutamic Acid Decarboxylase on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 05.21.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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