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Purified anti-URO-1 Antibody (Previously Covance catalog# SIG-38010)

Pricing & Availability
Clone
J143 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin alpha-3, FRP-2, galactoprotein B3, VLA-3 subunit alpha, CD49 antigen-like family member C, antigen identified by monoclonal antibody J143, very late activation protein 3 receptor, alpha-3 subunit
Previously
Signet Catalog# 8010-01
Covance Catalog# SIG-38010
Isotype
Mouse IgG1, κ
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Product Citations
publications
J143_Purified_URO-1_Antibody_IHC_032515
IHC staining of purified anti-URO-1 antibody (clone J143) on frozen human kidney tissue. The tissue was incubated with a 1:40 dilution of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • J143_Purified_URO-1_Antibody_IHC_032515
    IHC staining of purified anti-URO-1 antibody (clone J143) on frozen human kidney tissue. The tissue was incubated with a 1:40 dilution of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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917201 1 mL 322€
 
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Description

The alpha 3 subunit of VLA-3 consists of the ECMR I (extracellular matrix receptor I) and URO-1. VLA-3 is also known as CD49c and forms a heterodimer with β1 integrin for cell adhesion functions.  In tissues, the URO-1 antigen is expressed by glomeruli, the basal layer of transitional epithelium and skin, and the basement membrane of glands. URO-1 may serve as a helpful marker of certain tumors, as it has been found to be expressed in bladder tumors, most kidney and certain other epithelial tumors.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using the 253J Bladder cancer cell line.
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue section. For immunohistochemistry, a dilution of 1:40 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hemler M, et al. 1988. J Biol Chem. 263:7660.
  2. Cordon-Cardo C, et al. 1984. J Histochem Cytochem. (10): 1035-40. (IHC-F)
RRID
AB_2565195 (BioLegend Cat. No. 917201)

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Go To Top Version: 4    Revision Date: 02.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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