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Purified anti-RNA Polymerase II Rpb4 Antibody

Pricing & Availability
Clone
2Y14 (See other available formats)
Regulatory Status
RUO
Other Names
B32, DNA-directed RNA polymerase II 32 kD polypeptide, RNA polymerase II subunit B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
2Y14_PURE_RNA-Pol-II-Rpb4_Antibody_041620
Whole cell extracts (15 µg protein) from S. cerevisiae cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-RNA Polymerase II Rpb4 antibody (clone 2Y14) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 2Y14_PURE_RNA-Pol-II-Rpb4_Antibody_041620
    Whole cell extracts (15 µg protein) from S. cerevisiae cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-RNA Polymerase II Rpb4 antibody (clone 2Y14) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
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665105 25 µg 81€
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665106 100 µg 203€
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Description

Polymerase II (Pol II) is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2, and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp since RPB7 is in the closed conformation, which prevents double stranded DNA from entering the active site cleft. The RPB4-RPB7 subcomplex only binds single-stranded DNA and RNA. The RPB4-RPB7 subcomplex is necessary for promoter-directed transcription initiation, but is not required for recruitment of Pol II to active preinitiation complexes or transcription elongation and termination. The RPB4-RPB7 subcomplex recruits FCP1 to Pol II. This subcomplex is involved in DNA repairing of any damage in the transcribed strand. RPB4 is unnecessary in optimal growth conditions, but becomes essential during heat or cold shock and in nutrient depletion. It suppresses the RBP9-mediated transcription coupled repair (TCR) subpathway of nucleotide excision repair (NER), but facilitates the RAD26-mediated TCR subpathway. Under stress conditions, it is involved in mRNA export to the cytoplasm. RPB4 promotes or enhances the deadenylation process of specific mRNAs and may recruit PAT1 and the LSM1-7 complex to these mRNAs, stimulating their decapping and further decay.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Yeast
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

While the Rpb4 subunit of RNA polymerase II is evolutionarily conserved across eukaryotes, this antibody is not predicted to cross-react with Rpb4 from higher eukaryotes due to poor sequence conservation between the yeast Rpb4 immunogen and Rpb4 orthologs from other organisms.

Product Citations
  1. Baek I, et al. 2021. Molecular Cell. 81:3576. PubMed
RRID
AB_2861050 (BioLegend Cat. No. 665105)
AB_2861051 (BioLegend Cat. No. 665106)

Antigen Details

Structure
RPB4 is a 32 kD subunit of yeast RNA polymerase II.
Biology Area
Cell Biology, Transcription Factors
Gene ID
853301 View all products for this Gene ID
UniProt
View information about RNA Polymerase II Rpb4 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 09.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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