Purified anti-RING1 Antibody

Pricing & Availability
Clone
P88C12F4 (See other available formats)
Regulatory Status
RUO
Other Names
RING1A, RNF1, Ring Finger Protein 1, E3 Ubiquitin Ligase RING1, EC:2.3.2.27, Really Interesting New Gene 1 Protein, RNF1
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
P88C12F4_PURE_RING1_Antibody_1_042820
Whole cell lysates (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution) of purified anti-RING1 antibody (clone P88C12F4) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • P88C12F4_PURE_RING1_Antibody_1_042820
    Whole cell lysates (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution) of purified anti-RING1 antibody (clone P88C12F4) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
Cat # Size Price Quantity Check Availability Save
936701 25 µg 68,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
936702 100 µg 172,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

RING1 is an E3 ligase that is essential for development in higher eukaryotes and plants. It is a critical component of the Polycomb-group (PcG) multiprotein PRC1-like complex, which acts to transcriptionally repress genes through post- translational modifications that block the assembly of the transcriptional pre-initiation complex. As a component of PRC1, RING1 plays a pivotal role in monoubiquitination of Histone H2A at Lysine 119, which is linked to transcriptional repression of Hox family members.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human RING1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone may cross-react with RING1B/RING2.

?Due to this clone binding to additional proteins of unknown origin by western blot, it was not tested for ICC.

RRID
AB_2861119 (BioLegend Cat. No. 936701)
AB_2861120 (BioLegend Cat. No. 936702)

Antigen Details

Structure
RING1 is a 406 amino acid protein with a predicted molecular weight of 55 kD.
Distribution

RING1 expression is localized to the nucleus.

Function
Ubiquitin-protein ligase
Interaction
RING2/RNF2
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Ubiquitin/Protein Degradation
Molecular Family
Ligases
Antigen References
  1. Akasaka T, et al. 2002. Genes Cells. 7:835-50.
  2. Levine SS, et al. 2002. Mol Cell Biol. 22:6070-8.
  3. Lovering R, et al. 1993. Proc Natl Acad Sci USA. 90:2112-6.
  4. Satijn DP, et al. 1997. Mol Cell Biol. 17:4105-13.
  5. Wang H, et al. 2004. Nature. 431:873-8.
Gene ID
6015 View all products for this Gene ID
UniProt
View information about RING1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.28.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-RING1

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account