Purified anti-Lysozyme Antibody

Pricing & Availability
Clone
Poly28600 (See other available formats)
Other Names
Lysozyme, LZM, 1,4-Beta-N-Acetylmuramidase C, EC 3.2.1.17, Lysozyme C, Lysozyme (Renal Amyloidosis) 2
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly28600_Purified_Lysozyme_Antibody_1_051519.png
Western blot of purified anti-Lysozyme antibody (Poly28600). Lane 1: Molecular weight marker; Lane 2: 20 µg of human spleen lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28600_Purified_Lysozyme_Antibody_1_051519.png
    Western blot of purified anti-Lysozyme antibody (Poly28600). Lane 1: Molecular weight marker; Lane 2: 20 µg of human spleen lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28600_Purified_Lysozyme_Antibody_2_051519.png
    Western blot of purified anti-Lysozyme antibody (Poly28600). Lane 1: Molecular weight marker; Lane 2: 10 µg of rat kidney lysate; Lane 3: 20 µg of mouse kidney lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28600_Purified_Lysozyme_Antibody_3_051519.png
    IHC staining of purified anti-Lysozyme antibody (Poly28600) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28600_Purified_Lysozyme_Antibody_4_051519.png
    IHC staining of purified anti-Lysozyme antibody (Poly28600) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28600_Purified_Lysozyme_Antibody_5_051519.png
    IHC staining of purified anti-Lysozyme antibody (Poly28600) on formalin-fixed paraffin-embedded rat kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
860001 25 µg 76€
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Description

Lysozyme is an antimicrobial enzyme  that is abundantly present in secretions such as tears, saliva and mucus. Its hydrolase activity leads to the breakdown of the gram-positive bacterial cell wall and lysis of the bacteria.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Lysozyme expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml for human, and 1.0 - 2.0 µg per ml for mouse and rat. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Lysozyme is a 148 amino acid protein with an apparent molecular mass of ~ 16kD.
Distribution

Tissue Distribution: Spleen, kidney, milk, tears, and plasma
Cellular Distribution: Cytosol

Function
Lysozyme is expressed in many immune cells including monocytes and macrophages. It plays an important role in innate immunity by hydrolyzing peptidoglycans found in bacterial cell wall and thus destroying the bacteria.
Interaction
Bacterial peptidoglycan
Cell Type
Macrophages, Mesenchymal cells, Monocytes, Neutrophils
Biology Area
Bacterial proteins and Toxins, Cell Biology, Immunology, Innate Immunity
Molecular Family
Enzymes and Regulators, Innate Immune Signaling, Proteases
Antigen References
  1. Mallamace D, et al. 2018. Int J Mol Sci. 19(12): E3825.
  2. Delorme-Axford E and Klionsky DJ. 2018. Autophagy. 14(3):365.
Gene ID
4068 View all products for this Gene ID 17110 View all products for this Gene ID 25211 View all products for this Gene ID
UniProt
View information about Lysozyme on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05.15.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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