Purified anti-human Integrin α9β1 Antibody

Pricing & Availability
Clone
Y9A2 (See other available formats)
Other Names
ITGA9, ITGB1
Isotype
Mouse IgG1, κ
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Product Citations
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Y9A2_Pure_082911
Human peripheral blood granulocytes were stained with purified integrin α9β1 (clone Y9A2) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG-PE.
  • Y9A2_Pure_082911
    Human peripheral blood granulocytes were stained with purified integrin α9β1 (clone Y9A2) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG-PE.
Cat # Size Price Quantity Avail. Save
351602 100 µg 156€
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Description

Integrin α9β1 is a transmembrane protein belonging to the integrin family. It is widely expressed in airway epithelium, different types of muscle cells, and hepatocytes. In the hematopoietic system, integrin α9β1 is highly expressed on human neutrophils and at low level on monocytes. Multiple ligands have been identified for this integrin including the inducible endothelial counter-receptor, vascular cell adhesion molecule-1 (VCAM-1), the extracellular matrix proteins tenascin C and osteopontin, some members of ADAMs family, coagulation factor XIII, and von Willebrand factor. It has been reported that binding of the α9 cytoplasmic domain to spermidine/spermine N(1)-acetyltransferase (SSAT) mediates α9β1 enhancement of cell migration, mediates adhesion to the extracellular matrix proteins, and is involved in G-CSF signaling. More recently, integrin α9β1 was found on hematopoietic stem and progenitor cells to mediate stem cells adhesion, proliferation, and differentiation.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
L-cells transfected with human α9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for the relevant formats) include: immunofluorescence microscopy1, immunopricipitation2,  and blocking3.

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Lafuste P, et al. 2005. Mol. Biol. Cell. 16:861. (IF)
  2. Chen C, et al. 2006. Immunity 25:895. (FC, IP)
  3. Taooka Y, et al. 1999. J. Cell Biol. 145:413. (Block)
RRID
AB_10901165 (BioLegend Cat. No. 351602)

Antigen Details

Structure
Heterodimeric proteins made up of α9 and β1 subunits
Distribution

Neutrophil, monocytes, endothelial cells, hepatopoietic cells, and different type of muscle cells

Function
Receptor for VCAM-1, involved in cell adhesion and migration
Interaction
Recognizes α9 integrin expressed as a heterodimer in association with the β1 integrin.
Ligand/Receptor
VCAM-1, ECM proteins tenascin C, osteopontin
Cell Targets
Binds VCAM-1
Cell Type
Endothelial cells, Monocytes, Neutrophils
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Schreiber TD, et al. 2009 Haematologica 94:1495.
2. Chen C, et al. 2006 Immunity 25:895.
3. Taooka Y, et al. 1999 J. Cell Biol. 145:413.
4. Palmer EL, et al. 1993 J. Cell Biol. 123:1289.

Gene ID
3680 View all products for this Gene ID 3688 View all products for this Gene ID
UniProt
View information about Integrin alpha9 beta1 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05.29.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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