Purified anti-DNA-PKcs Phospho (Thr2609) Antibody

Pricing & Availability
Clone
10B1 (See other available formats)
Other Names
DNA-protein kinase catalytic subunit, DNA-activated protein kinase, DNA-dependent protein kinase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
10B1_PURE_DNA-PKcs_Antibody_1_IF_012316
3 minutes UV treated HeLa cells (recovered 30 minute) were fixed with 4% paraformaldehyde (PFA) and then were permeabilized with 0.5% Triton X-100 followed by blocking with 5% FBS. The cells were then intracellularly stained with 5 µg/ml anti-DNA-PKcs phospho (Thr2609) (clone 10B1) antibody in blocking buffer overnight at 4°C followed by staining with Alexa Fluor® 488 conjugated goat anti-mouse IgG antibody (clone Poly4053) for one hour at room temperature. The images were captured with a 40X objective.
  • 10B1_PURE_DNA-PKcs_Antibody_1_IF_012316
    3 minutes UV treated HeLa cells (recovered 30 minute) were fixed with 4% paraformaldehyde (PFA) and then were permeabilized with 0.5% Triton X-100 followed by blocking with 5% FBS. The cells were then intracellularly stained with 5 µg/ml anti-DNA-PKcs phospho (Thr2609) (clone 10B1) antibody in blocking buffer overnight at 4°C followed by staining with Alexa Fluor® 488 conjugated goat anti-mouse IgG antibody (clone Poly4053) for one hour at room temperature. The images were captured with a 40X objective.
  • 10B1_PURE_DNA-PKcs_Antibody_2_IF_012316
    Untreated HeLa cells were fixed with 4% paraformaldehyde (PFA) and then were permeabilized with 0.5% Triton X-100 followed by blocking with 5% FBS. The cells were then intracellularly stained with 5 µg/ml anti-DNA-PKcs phospho (Thr2609) (clone 10B1) antibody in blocking buffer overnight at 4°C followed by staining with Alexa Fluor® 488 conjugated goat anti-mouse IgG antibody (clone Poly4053) for one hour at room temperature. The images were captured with a 40X objective.
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Cat # Size Price Quantity Avail. Save
612901 25 µg 80€
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612902 100 µg 200€
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Description

DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damaage. The 10B1 monoclonal antibody recognizes phosphorylated human DNA-PKcs (Thr2609) and has been shown to be useful for immunofluorescence staining and Western blotting.

Product Details
Technical data sheet

Product Details

Reactivity
Human, reacts with Thr2609-phosphorylated DNA-PKcs
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Modified peptide
Formulation
This DNA-PKcs antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Eid M, et al. 2014. J Immunol. 192:5529. PubMed
Publication Library
RRID
AB_315784 (BioLegend Cat. No. 612901)
AB_315785 (BioLegend Cat. No. 612902)

Antigen Details

Structure
PI3/PI4-Kinases family. Spliced products 460 kD, 470 kD
Distribution

Nuclear

Function
Serine/Threonine kinase, dsDNA break repair, VDJ recombination, transcriptional modulator
Interaction
Ku70/Ku80, KIP, DNA-ligase IV, XRCC4
Modification
Phosphorylation
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Signal Transduction, Transcription Factors
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References

1. Hartley K, et al. 1995. Cell. 82:849.
2. Connelly M, et al. 1996. Gene. 175:271.
3. Douglas P, et al. 2002. Biochem. J. 368:243
4. Calsou P, et al. 2003. J. Mol. Biol. 326:93.

Regulation
Must bind DNA ends to become active
Gene ID
5591 View all products for this Gene ID
UniProt
View information about DNA-PKcs Phosphorylated on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 11.15.2017

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