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PerCP/Cyanine5.5 anti-mouse/rat/human CD27 Antibody

Pricing & Availability
Clone
LG.3A10 (See other available formats)
Regulatory Status
RUO
Other Names
T14, S152, Tp55, TNFRSF7
Isotype
Armenian Hamster IgG
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Product Citations
publications
LGdot3A10_PerCPCyanine55_CD27_Antibody_091819.png
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC and CD27 (clone LG.3A10) PerCP/Cyanine5.5 (left) or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • LGdot3A10_PerCPCyanine55_CD27_Antibody_091819.png
    C57BL/6 mouse splenocytes were stained with CD45R/B220 APC and CD27 (clone LG.3A10) PerCP/Cyanine5.5 (left) or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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124213 25 µg 95€
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124214 100 µg 227€
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Description

CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, it is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat, Human
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA
Formulation
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: functional assay1,3 and immunohistomecial staining of acetone-fixed frozen sections2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 124243 & 124244).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Gravestein LA, et al. 1995. Int. Immunol. 7:551. (FA)
  2. Gravestein LA, et al. 1996. J. Exp. Med. 184:675. (IHC)
  3. Takeda K, et al. 2000. J. Immunol. 164:1741. (FA)
  4. Welner RS, et al. 2009. J. Immunol. 183:7768. PubMed
  5. Vicetti Miguel RC, et al. 2012. J. Immunol. 189:3449. PubMed
  6. White CA, et al. 2014. J Immunol. 193:5933. PubMed
  7. Iwata S, et al. 2015. Lupus. 24:695. PubMed
Product Citations
  1. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  2. Wang D, et al. 2018. Immunity. 48:659. PubMed
  3. Sandrock I, et al. 2015. PLoS One. 10: 0145010. PubMed
  4. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  5. Gu X, et al. 2022. Vaccines (Basel). 10:. PubMed
  6. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  7. Colston J, et al. 2016. J Immunol. 196: 3354-3363. PubMed
  8. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  9. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  10. Topham H 2010. J Virol. 84:7750. PubMed
  11. S Tzeng 2016. J Vis Exp. 118:e54582. PubMed
  12. Bantug GR, et al. 2018. Immunity. 48:542. PubMed
  13. Rissiek B, et al. 2014. J Leukoc Biol. 95:543. PubMed
  14. Forconi F, et al. 2010. Blood. 115:71. PubMed
RRID
AB_2073424 (BioLegend Cat. No. 124213)
AB_2275577 (BioLegend Cat. No. 124214)

Antigen Details

Structure
TNFR superfamily, 45 kD
Distribution

Peripheral blood T cells, memory B cells, NK cells, thymocyte subset

Function
Costimulatory signal for T and B cell activation, T cell development
Interaction
TRAF2, TRAF5, CD27-binding protein (Siva)
Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hintzen RQ, et al. 1994. Immunol. Today 15:307.
2. Akiba H, et al. 1998. J. Biol. Chem. 273:13353.
3. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249.
4. Hendriks J, et al. 2000. Nat. Immunol. 1:433
5. Hintzen RQ, et al. 1994. Int. Immunol. 6:477
6. Camerini D, et al. 1991. J. Immunol. 147:3165

Gene ID
21940 View all products for this Gene ID 939 View all products for this Gene ID 500318 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 09.18.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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