- 561 (See other available formats)
- Regulatory Status
- Other Names
- Gp105-120, My10, Mucosialin
- Mouse IgG2a, κ
- Ave. Rating
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- Product Citations
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CD34, also known as gp105-120, is a type I monomeric sialomucin-like glyco-phosphoprotein with an approximate molecular weight of 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. Based on different sensitivities, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.Product Details
- Verified Reactivity
- Reported Reactivity
- Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
The 561 antibody recognizes a class III group epitope, which is resistant to sialidase/glycolyprotease and chymopapain treatment.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82.
- Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
- Product Citations
AB_2566787 (BioLegend Cat. No. 343611)
AB_2566788 (BioLegend Cat. No. 343612)
- 105-120 kD single chain mucin-like glycoprotein
Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
- Cell adhesion
- L-selecin, E-selectin
- Cell Type
- Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- CD Molecules
- Gene ID
- 947 View all products for this Gene ID
- View information about CD34 on UniProt.org
Related Pages & Pathways
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other FormatsView All CD34 Reagents Request Custom Conjugation
|Purified anti-human CD34||561||FC|
|FITC anti-human CD34||561||FC|
|PE anti-human CD34||561||FC|
|APC anti-human CD34||561||FC|
|Brilliant Violet 421™ anti-human CD34||561||FC|
|PerCP/Cyanine5.5 anti-human CD34||561||FC|
|APC/Cyanine7 anti-human CD34||561||FC|
|PE/Cyanine7 anti-human CD34||561||FC|
|Alexa Fluor® 647 anti-human CD34||561||FC|
|Brilliant Violet 650™ anti-human CD34||561||FC|
|Brilliant Violet 785™ anti-human CD34||561||FC|
|Alexa Fluor® 488 anti-human CD34||561||FC|
|Alexa Fluor® 700 anti-human CD34||561||FC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD34
FITC anti-human CD34
PE anti-human CD34
APC anti-human CD34
Brilliant Violet 421™ anti-human CD34
PerCP/Cyanine5.5 anti-human CD34
APC/Cyanine7 anti-human CD34
PE/Cyanine7 anti-human CD34
Alexa Fluor® 647 anti-human CD34
Brilliant Violet 650™ anti-human CD34
Brilliant Violet 785™ anti-human CD34
Alexa Fluor® 488 anti-human CD34
Alexa Fluor® 700 anti-human CD34