PE anti-mouse CD1d Antibody

Pricing & Availability
K253 (See other available formats)
Regulatory Status
Other Names
Antigen-presenting glycoprotein CD1d1, CD1.1, Ly-38
Mouse IgG1, κ
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Product Citations
C57BL/6 splenocytes stained with K253 PE
  • K253_PE_082610
    C57BL/6 splenocytes stained with K253 PE
See PE spectral data
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140805 50 µg 79€
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CD1d is a type I transmembrane protein and member of the MHC family, with a molecular weight ranging from 43-49 kD, depending on the glycosylation degree. CD1d is expressed by antigen presenting cells such as dendritic cells, monocytes, macrophages and B cells; also expressed by thymocytes and intestinal epithelial cells. CD1d present glycolipids to iNKT cells that recognize them by their Vα14i TCR.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
α-GalCer:mCD1d complexes coupled to Mycobacterium tuberculosis PPD
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA1, immunofluorescence2 to detect CD1d in the lipid rafts on the cell membrane, and block1 CD1d recognition of an iNKT cell hybridoma.

Application References

(PubMed link indicates BioLegend citation)
  1. Yu KO, et al. 2007. J. Immunol. Methods 323:11. (ELISA, Block)
  2. Im JS, et al. 2009. Immunity 30:888. (IF)
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
AB_10643277 (BioLegend Cat. No. 140805)

Antigen Details

Member of the MHC family. Type I transmembrane protein. Associates with β2-microglobulin. Molecular weight ranges from 43 - 49 kD, depending on glycosylation.
Dendritic cells, monocytes, macrophages, B cells, thymocytes, intestinal epithelial cells
Glycolipid presentation to iNKT cells
Vα14i TCR
Cell Type
Dendritic cells, Monocytes, Macrophages, B cells, T cells, Epithelial cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.

Gene ID
12479 View all products for this Gene ID
View information about CD1d on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-mouse CD1d

  • PE anti-mouse CD1d


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