- K253 (See other available formats)
- Regulatory Status
- Other Names
- Antigen-presenting glycoprotein CD1d1, CD1.1, Ly-38
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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CD1d is a type I transmembrane protein and member of the MHC family, with a molecular weight ranging from 43-49 kD, depending on the glycosylation degree. CD1d is expressed by antigen presenting cells such as dendritic cells, monocytes, macrophages and B cells; also expressed by thymocytes and intestinal epithelial cells. CD1d present glycolipids to iNKT cells that recognize them by their Vα14i TCR.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- α-GalCer:mCD1d complexes coupled to Mycobacterium tuberculosis PPD
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: ELISA1, immunofluorescence2 to detect CD1d in the lipid rafts on the cell membrane, and block1 CD1d recognition of an iNKT cell hybridoma.
(PubMed link indicates BioLegend citation)
- Yu KO, et al. 2007. J. Immunol. Methods 323:11. (ELISA, Block)
- Im JS, et al. 2009. Immunity 30:888. (IF)
- Product Citations
AB_10643277 (BioLegend Cat. No. 140805)
- Member of the MHC family. Type I transmembrane protein. Associates with β2-microglobulin. Molecular weight ranges from 43 - 49 kD, depending on glycosylation.
- Dendritic cells, monocytes, macrophages, B cells, thymocytes, intestinal epithelial cells
- Glycolipid presentation to iNKT cells
- Vα14i TCR
- Cell Type
- Dendritic cells, Monocytes, Macrophages, B cells, T cells, Epithelial cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, TCRs
- Antigen References
1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.
- Gene ID
- 12479 View all products for this Gene ID
- View information about CD1d on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
|Purified anti-mouse CD1d||K253||FC,Block,ELISA,ICC|
|PE anti-mouse CD1d||K253||FC|
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