PE anti-human Basophils (2D7) Antibody

Pricing & Availability
Clone
Basophil/2D7 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
Basophil2D7_PE_1_022212
Human peripheral blood mononuclear cells were stained with CCR3 APC, fixed, permeabilized and then stained with Basophil/2D7 PE (top) or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on the lymphoid cell population.
  • Basophil2D7_PE_1_022212
    Human peripheral blood mononuclear cells were stained with CCR3 APC, fixed, permeabilized and then stained with Basophil/2D7 PE (top) or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on the lymphoid cell population.
  • Basophil2D7_PE_2_022212
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
346206 100 tests 278€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The Basophil/2D7 mAb specifically recognizes the human basophil population. The antigen that this antibody binds is present in cell granules but its identity is still unknown.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Lysates of purified basophils
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications for the relevant formats include: immunofluorescence staining7, and immunohistochemistry of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1-6.

Application References

(PubMed link indicates BioLegend citation)
  1. Kepley CL, et al. 1995. J. Immunol. 154:6548. (IHC)
  2. Irani AM, et al. 1998. J. Allergy Clin. Immunol. 1998 101:354. (IHC)
  3. Agis H, et al. 2006. J. Clin. Pathol. 59:396. (IHC)
  4. Plager DA, et al. 2006. J. Allergy Clin. Immunol. 117:626. (IHC)
  5. Sperr WR, et al. 2001. Blood 98:2200. (IHC)
  6. Aichberger KJ, et al. 2006. Blood 108:3538. (IHC)
  7. Kepley CL, et al. 1998. J. Allergy Clin. Immun. 102:304. (IF)
RRID
AB_11125171 (BioLegend Cat. No. 346206)

Antigen Details

Distribution

Basophils.

Cell Type
Basophils
Biology Area
Immunology, Innate Immunity
Gene ID
NA
UniProt
View information about Basophil on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 05.31.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account