LEGEND MAX™ Human Myeloperoxidase ELISA Kit

Pricing & Availability
Other Names
MPO, Myeloperoxidase
Ave. Rating
Submit a Review
Product Citations
publications
MPO_LegMax_ELISA_012413
  • MPO_LegMax_ELISA_012413
Cat # Size Price Quantity Save
440007 1 Pre-coated Plate 316€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Myeloperoxidase (MPO) is a peroxidase enzyme which is expressed in neutrophils and macrophages. MPO is a 150 kD dimeric protein which consists of two 15 kD light chains and two variable weight glycosylated heavy chains bound to a prosthetic heme group. Three isoforms have been identified, differing in the size of the heavy chain. Mature human MPO shares 88% amino acid sequence identity with dog, mouse, and rat. Human MPO also shares 71%, 56%, and 47% amino acid sequence identity with comparable regions of human eosinophil peroxidase, lactoperoxidase, and thyroid peroxidase, respectively.

MPO is released extracellularly, and it has been detected in a wide range of acute and chronic inflammatory conditions. Serum levels of myeloperoxidase (MPO) are associated with coronary artery disease and indicate a risk factor for pancreatic cancer.

The LEGEND MAX™ Human Myeloperoxidase ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) is a 96-well strip plate, pre-coated with a monoclonal capture antibody. This kit is specifically designed for the accurate quantitation of human MPO from cell culture supernatant, serum, plasma, saliva, urine and other biological fluids. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Human MPO Pre-coated 96-well Strip Microplate
  • Human MPO Dectection Antibody
  • Human MPO Standard
  • Avidin-HRP D
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human
Application

ELISA

Product Citations
  1. Hertwig L, et al. 2016. Mult Scler. 22: 160 - 173. PubMed
Publication Library
Sensitivity
28 ± 8 pg/mL
Standard Range
0.16-10 ng/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Biology Area
Cancer Biomarkers, Cell Biology
Molecular Family
Enzymes and Regulators
Gene ID
4353 View all products for this Gene ID
UniProt
View information about MPO on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 2    Revision Date: 09.26.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login / Register
Forgot your password? Reset password?
Create an Account