HRP Goat anti-rat IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4054 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
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Product Citations
publications
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405405 500 µL 113€
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Description

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The polyclonal antibody was purified from goat antiserum by rat immunoglobulin affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product
Application

ELISA - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. Do not use this product beyond the expiration date. For ELISA or Western blot analysis, the reagent should be titrated between 1:1,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.

Application Notes

This conjugated goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.

Additional Product Notes

This HRP conjugated goat anti-rat IgG antibody is useful as a second step reagent for indirect immunohistochemistry staining when used in conjunction with primary antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
  2. Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
  3. Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations
  1. Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
  2. Malik TH, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  3. Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed
  4. van Tok MN, et al. 2018. Front Immunol. 9:1550. PubMed
  5. Baba T, et al. 2021. Cell Death Dis. 12:322. PubMed
  6. Domenger A, et al. 2022. EMBO Mol Med. 14:e14740. PubMed
  7. Mamedov T, et al. 2021. Front Plant Sci. 12:742875. PubMed
  8. Kim DH, et al. 2018. Nat Commun. 9:503. PubMed
  9. Sahlberg S, et al. 2014. PLoS One. 9:94621. PubMed
  10. Lou Q, et al. 2019. J Immunother Cancer. 7:210. PubMed
  11. Snelling T, et al. 2022. Biochem J. :. PubMed
  12. Callejas BE, et al. 2021. Front Immunol. 12:744738. PubMed
  13. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  14. Lu SW, et al. 2020. Nat Commun. 3.660416667. PubMed
  15. Link E, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.08.019. PubMed
  16. Freidl R, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31348-3. PubMed
  17. Tuncel J, et al. 2016. PLoS One. 11: 0155936. PubMed
  18. Mamedov T, et al. 2019. Sci Rep. 9:9868. PubMed
  19. Hayashi M, et al. 2021. Viruses. 13:. PubMed
  20. Siddiqui S, et al. 2016. J Biol Chem. 292:1029-1037. PubMed
RRID
AB_315016 (BioLegend Cat. No. 405405)

Related FAQs

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Go To Top Version: 5    Revision Date: 12.12.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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